1a.Objectives (from AD-416):
The objective of this agreement is to work together to conduct studies examining the effects of phytochemicals on cancer cells and cancer stem cells. Studies will focus on genes important in the process of carcinogenesis.
1b.Approach (from AD-416):
ARS will use cell culture models of prostate cancer cells or prostate cancer stem cells to test the effects of diet-derived phytochemicals on their ability to inhibit carcinogenesis. The focus will be on cellular pathways that may be important in the carcinogenesis process, including steroid hormone-related pathways, xenobiotic metaboism, etc.
Soy-derived glyceollins are novel bioactive compounds. The effects on the androgen responsive genes PSA and NKX3.1 were tested in the cell model. Significant inhibition of PSA expression was observed in cells treated with 1 µM or higher concentration of individual glyceollins (I, II, III). At a concentration of 5 µM, glyceollins reached its maximum inhibitory effect on PSA expression. Individual glyceollins started to show an inhibitory effect on NKX3.1 expression at 0.5 µM and reached maximum inhibition at 5 µM. These results indicated a dose-dependent down-regulation of androgen responsive genes. Also, individual glyceollins possess a similar inhibitory effect on androgen responsive genes.