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United States Department of Agriculture

Agricultural Research Service

Research Project: R-GENE CLUSTERS FOR PHYTOPHTHORA SOJAE RESISTANCE: CLONING RPS GENES

Location: Corn Insects and Crop Genetics Research

Project Number: 3625-21220-004-23
Project Type: Reimbursable

Start Date: Jan 01, 2012
End Date: Dec 31, 2012

Objective:
1. Sequence the Rps2, Rps3 and Rps8 loci from the resistant parents and use expression data to identify expressed candidate resistance genes. 2. Use virus induced gene silencing (VIGS) to assay the function of candidate resistance genes in response to Phytopthora sojae infection.

Approach:
Our approach will identify candidate Phytophthora sojae resistance genes for Rps2, Rps3 and Rps8. We will leverage the Williams82 and PI 96983 genome sequences and use it to identify candidate genes from the resistant parents. Our approach will be to compare sequence from the Williams82 soybean genome and sequence from PI96983, which does not have an Rps gene in these regions, to sequence from lines with Rps2, Rps3 and Rps8. Real-time PCR will be used to monitor expression of candidate genes in resistant and susceptible parents following P. sojae infection. The function of expressed candidate resistance genes will be assayed using virus induced gene silencing to "turn off" resistance.

Last Modified: 7/31/2014
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