1a.Objectives (from AD-416):
The objective is to transform wheat with a chimeric gene for transgenic resistance to the Hessian fly.
1b.Approach (from AD-416):
The chimeric construct will be used to transform wheat for potential transgenic resistance to Hessian fly and other major insect pests of wheat (i.e. greenbug, Russian wheat aphid).
A chimeric gene expressing a fusion protein of snowdrop lectin and an insect specific toxin ButaIT was obtained by the USDA-ARS Hessian fly research project from Durham University, UK. This transgene was used by the collaborator at Kansas State University to transform wheat. Seed for transgenic wheat carrying the chimeric transgene was then sent to the USDA-ARS Hessian fly research project at West Lafayette, IN where a transgenic line stably expressing the transgene is being selected. Once this line has been selected it will be tested for efficacy of resistance against the major insect pest of wheat (i.e. Hessian fly, greenbug, Russian wheat aphid, and wheat stem sawfly).