Characterizing the Transcriptome of Endophytic Trichoderma Isolates
Sustainable Perennial Crops
2012 Annual Report
1a.Objectives (from AD-416):
The objective of this research is to test in the laboratory and growth chambers, the efficacy of selected Trichoderma isolates in colonizing seedlings of annual plant species and evaluating the isolates’ abilities to induce plant defense responses and reduce plant disease. ARS is studying the same effects in the perennial tree species Theobroma cacao (cacao) and comparisons between the interactions observed in different plant species is of special interest. The Trichoderma isolates to be studied were collected in tropical environment where cacao is grown and little information exist concerning how the Trichoderma isolates perform in annual plant species. The data gathered will answer questions as to the uniqueness of the Trichoderma/cacao interaction and potentially broaden the number of cropping systems any Trichoderma based products developed might be applicable too. The latter aspect may be critical to successfully marketing Trichoderma based products for management of diseases in cacao in addition to other crops.
1b.Approach (from AD-416):
ARS is carrying out transcriptome analysis for Trichoderma species endophytic on cacao. The Trichoderma/cacao interaction is considered unique in that the Trichoderma colonizes the above ground plant parts and not just roots. In order to identify Trichoderma transcript expression patterns specific to the Trichoderma/cacao interaction we need to know the expression pattern of Trichoderma in other crops. The Cooperator will inoculate pepper, cotton, corn, and wheat with Trichoderma isolates endophytic on cacao. Trichoderma colonization of roots and stems will be measured. RNA will be isolated from roots and stems of annual species and Trichoderma gene expression studied in these tissues. The data gathered will be compared to data gathered by ARS on the Trichoderma/cacao interaction. Growth chamber assays will be carried out evaluating the ability of Trichoderma isolates to reduce disease in pepper (Capsicum annuum) caused by Phytophthora capsici. Depending on results from the transcript analysis, additional disease assays will be carried out on other plant/pathogen systems.
To carry out this project, our collaborator is acquiring the APHIS permits required for exchange and use of tropical Trichoderma isolates in New Mexico. Once the permits are acquired we will be able to carry out the proposed experiments involving crops traditionally grown in the United States.