Location: Sustainable Perennial Crops
Project Number: 8042-21220-252-22
Start Date: Aug 15, 2011
End Date: Aug 15, 2016
ARS is carrying out transcriptome analysis for Trichoderma species endophytic on cacao. The Trichoderma/cacao interaction is considered unique in that the Trichoderma colonizes the above ground plant parts and not just roots. In order to identify Trichoderma transcript expression patterns specific to the Trichoderma/cacao interaction we need to know the expression pattern of Trichoderma in other crops. The Cooperator will inoculate pepper, cotton, corn, and wheat with Trichoderma isolates endophytic on cacao. Trichoderma colonization of roots and stems will be measured. RNA will be isolated from roots and stems of annual species and Trichoderma gene expression studied in these tissues. The data gathered will be compared to data gathered by ARS on the Trichoderma/cacao interaction. Growth chamber assays will be carried out evaluating the ability of Trichoderma isolates to reduce disease in pepper (Capsicum annuum) caused by Phytophthora capsici. Depending on results from the transcript analysis, additional disease assays will be carried out on other plant/pathogen systems.