1a.Objectives (from AD-416):
To determine how salmon consumption alters lipid oxidation parameters in human plasma. In this work, we will quantify the content of omega-6 and omega-3 fatty acids in the plasma from participants in the GFHNRC human study that have consumed differing amounts of salmon as a source of omega-3 fatty acids. These data will provide information on the role of fish consumption upon markers of inflammation and oxidative stress. Attainment of this data may be useful in future deliberations of the dietary guidelines committee on the evidence base for long chain omega-3 fatty acid recommended intake levels.
1b.Approach (from AD-416):
The GFHNRC will perform the necessary salmon feeding trial and collection of plasma samples. Samples of plasma will be delivered to the cooperator for analysis of fatty acid oxidation products. The cooperator will also offer expertise in interpretation and writing of the results. Data will be presented and published with GFHNRC scientists and the cooperator as authors. The cooperator will also offer expertise in interpretation of the results of the phospholipid fatty acids.
During the past year, the GFHNRC completed the necessary salmon feeding trial in people and collection of plasma samples. The cooperator analyzed the plasma for content of fatty acids in the plasma phospholipids. The cooperator offered expertise in interpretation and writing of the results. Data were presented at the Experimental Biology meeting April 2012. The results are currently under submission with GFHNRC scientists and the cooperator as authors.