IDENTIFICATION OF THE SPECIFIC AMINO ACIDS OF ARA H 2/6 THAT ARE MODIFIED DURING THERMAL PROCESSING OF PEANUTS
Project Number: 6435-43440-044-06
Specific Cooperative Agreement
Start Date: Aug 01, 2011
End Date: Dec 31, 2013
To clone and express Ara h 2 and Ara h 6 in soluble form, and to subject these clones to various chemical treatments known to occur during processing, in order to identify specific modifications.
a.) Clone Ara h 2/Ara h 6 into various types of DNA vectors towards making soluble protein. b.) Purify this allergen and subject the purified allergen to processing induced modification towards identification of specific amino acids involved in enhanced IgE binding following roasting.