1a.Objectives (from AD-416):
To determine mode of inheritance of beet curly top ("BCT") disease and conduct molecular genetic analysis using DNA markers using seed supplied by Cooperator SESVanderHave. To investigate BCT virus accumulation in populations using ELISA.
1b.Approach (from AD-416):
The cooperator will provide seeds for two sugar beet populations segregating for beet curly top. The ARS will grow the two populations in the greenhouse and individually inoculate the plants with viruliferous hoppers. The cooperator and ARS will collect leaf samples for DNA analysis and ELISA. The ARS and the cooperator’s scientist will collect phenotypic data. Data analysis will be conducted by both parties.
This cooperation focuses on studying the inheritance of curly top in different genetic backgrounds and populations. This research goal is to advance knowledge related to genetics of BCT disease from the parent project. The project progressed as planned to date. Seeds were received from the cooperator and planted in the greenhouse (June 2011). At appropriate growth stage the two populations were inoculated with BCT virus using viruliferous hoppers with clip-cage method. The two populations were phenotyped for BCT symptoms. Additionally, ELISA test was conducted on samples from all the individuals of the two populations. The cooperator used molecular markers for genotyping the two populations. The phenotypic and ELISA data were analyzed and shared between the parties. A genetic linkage map was constructed from one population that provided preliminary knowledge on major gene controlling BCT. Other chromosomal regions showed some effect on regulating the trait. One population phenotypic data was skewed and did not reveal map position. This population will be phenotyped, and genotyped again (June 2012).
The collaboration is monitored through direct visits between parties that visited SESVanderHave and shared results during the visit and discussed progress and future plans. The SESVanderHave scientists visited the greenhouse during the evalution of the population and will visit this year as well.