Location: Vegetable Crops Research Unit
2012 Annual Report
2. Combine genomic tools, segregating populations, and phenotypic characterization to identify loci associated with fruit size. Genetic analysis using the SNP arrays developed in this project and segregating populations for fruit size will identify QTL associated with fruit size.
Development of an integrated genetic-physical map. A high-density linkage map for cucumber was developed with simple sequence repeat (SSR) markers. The completeness of this linkage map was verified with fluorescence in situ hybridization (FISH) in the pachytene stage.
Quantitative trait loci (QTL) mapping of fruit size was conducted using F3 fruit size data and the linkage mapping data. Three QTLs were identified for fruit length and diameter. Deoxyribonucleic acid (DNA) is being extracted from F7/F8. RILs will be sent to collaborators for single-nucleotide polymorphism (SNP) genotyping.
This research relates to Objective 1, Determine the genetic basis of and initiate selection for carrot, onion, cucumber, and melon quality attributes influencing human nutrition and health, disease resistances, and yield and quality components, and stress tolerance in cucurbits, and perform field performance and quality trials; Objective 2, Utilize current biotechnology to discover and evaluate genetic variation and to map agriculturally important traits in Allium, Cucurbit, and Daucus germplasm, and to develop genetic and breeding stocks.