Project Number: 1910-21220-004-28
Project Type: Trust

Start Date: May 01, 2009
End Date: Dec 31, 2012

Objective:
Knock down candidate susceptiblity genes in transgenic grapes and assay disease resistance; conduct ecoTILLING in the vinifera germplasm collection and self-hybridize susceptible heterozygotes.

Approach:
We have identified orthologous candidate genes shown to be required for susceptiblity to Arabidopsis powdery mildew and are cloning these genes into silencing constructs. We will outsource the stable transformation of susceptible grapes with these constructs and test their resistance. Transformations will be conducted at the Ralph M. Parsons Plant Transformation Facility.