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United States Department of Agriculture

Agricultural Research Service

Research Project: Using Biochemistry and Biophysics to Investigate the Structure and Function of the Sorghum 4-Coumarate Coa Ligase

Location: Grain, Forage & Bioenergy Research

Project Number: 5440-21220-032-06
Project Type: Nonfunded Cooperative Agreement

Start Date: Jun 01, 2011
End Date: May 31, 2016

Objective:
Solve the crystal structure of the sorghum 4-coumarate CoA ligase and investigate interactions between the enzyme and its bound substrates.

Approach:
This will be a joint effort between ARS, who has sorghum 4-coumarate CoA ligases in a protein expression vector, expertise to produce, and assay protein activity, and the Washington State University, who has facilities and expertise to crystallize and solve the crystal structure of the protein. Both partners will collaborate in designing the experiments and interpreting the results.

Last Modified: 8/22/2014
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