Improving the resolution of the ovine linkage map and identifying genetic mutations associated with production traits
Range Sheep Production Efficiency Research
2013 Annual Report
1a.Objectives (from AD-416):
1) Increase the resolution of the sheep linkage map.
2) Identify genomic region(s) associated with phenotype(s) relevant to sheep producers.
1b.Approach (from AD-416):
Blood or tissue samples have been collected and stored for all sheep breeds at the U.S. Sheep Experiment Station (USSES). DNA will be extracted from samples of a subset of related animals. Genotypes will be generated using the Ovine SNP50 BeadChip. Pedigree data will be analyzed with the genotypes to improve the ovine linkage map resolution.
Generating Ovine SNP50 BeadChip genotypes will create the opportunity to perform genome-wide association studies. Thus, production-relevant sheep phenotype(s) will be evaluated with the genotypes to determine whether the phenotype(s) are statically associated with region(s) of the sheep genome.
The original principle investigator on this project is no longer with the location. As such, the agreement will terminate at the end of FY2013. Over the life of the agreement, U. S. Sheep Experiment Station scientists provided contributed sheep DNA samples for genotyping with an ovine single-nucleotide polymorphism (SNP) marker set and pedigrees of the genotyped sheep. Based on the outcome, the collaborator was able to increase marker density and refine marker position, which, in turn, will improve scientists' ability to identify genes, underlying quantitative-trait loci (QTL), and improve map comparisons between sheep and other animals. This collaboration supported Objective 2 of the parent project, which is focused on improving maternal and paternal genetic lines of sheep to best match western rangeland environments.