1a.Objectives (from AD-416):
Investigate the presence and abundance of novel starch biosynthesis pathway genes in wild sorghums.
1b.Approach (from AD-416):
This will be a joint effort between ARS, who has wild sorghum genetic materials in their National Collection from throughout the world, and expertise to produce, harvest, and preserve DNA samples from those materials, and the University of Queensland, who has facilities and expertise to evaluate such DNA samples for presence and abundance of unique biosynthesis pathway genes. The University of Queensland will also gather similar data on genetic materials collected in Australia. Both partners will collaborate in designing the experiments and interpreting the results.
This project is investigating the presence and abundance of novel starch biosynthesis pathway genes in wild sorghums. Dried plant materials were provided from 96 wild and weedy sorghum subspecies from the U.S. germplasm collection for DNA extraction. A third party performed next-generation DNA sequencing on isolated DNA. Analysis of the genes involved in starch metabolism is ongoing. This research will provide critical information on how starch synthesis was altered during the domestication of sorghum.