Location: Crop Improvement & Utilization Research
Project Number: 5325-21000-020-03
Start Date: Sep 27, 2011
End Date: Aug 31, 2015
The proposed research will develop the platforms needed to use two novel pairs of unidirectional recombinase enzymes, namely Bxb 1 and ParA, for performing precise genetic engineering of crops. These recombinases can perform controlled DNA integration into or excision from plant chromosomes based on the presence and orientation of specific recognition target sites. Agrobacterium-mediated transformation will be used to generate multiple candidate founder transgenic lines in which the recombination platforms can be tested and shown to function for generating transgenic plants with valuable new traits and lacking superfluous foreign DNA. Camelina, a bioenergy crop, will be used as a test system for the recombination platform because its transformation system is quicker. Then the recombination platform will be introduced into soybean and citrus. In addition, to improve the healthfulness of citrus fruit, citrus genes encoding enzymes necessary for anthocyanin and lycopene production will be isolated and expressed using promoters that are shown to be fruit-specific.