Location: Plant Genetics Research
Project Number: 5070-21000-036-02
Start Date: Apr 01, 2011
End Date: Mar 31, 2014
Molecular genetics and plant breeding experiments will be conducted to evaluate the molecular genetics of protein content and quality in the seeds. An established population will be used, so phenotyping and mapping can be completed efficiently. Trait combination will be assisted with developed and new molecular marker assays. Seeds will be phenotyped for their oil and protein contents by NIR or by NMR. Amino acid analyses will be performed in house using an existing method for HPLC or contracted out, depending on preliminary experimental results. DNA will be prepared from each of approximately 300 lines of the population and used for associating the high protein trait with the components of the genome responsible for the trait utilizing the Illumina Golden Gate Array in conjunction with ARS researchers in Beltsville, MD. Yield of selected lines from the mapping population will be analyzed in multiple Missouri field locations. Conventional plant breeding utilizing crossing and selection with existing molecular markers will be used to combine the most promising high protein quantity/quality lines trait lines with the low phytate and low P34 allergen traits.