2011 Annual Report 1a.Objectives (from AD-416) Increase protein contents, improve amino acid profile, and decrease phytate in MG-II to IV by incorporating appropriate genes from diverse sources of soybean lines. 1b.Approach (from AD-416) The objective will be achieved by making crosses with high-protein, low phytate sources from other state cooperators to elite soybean lines and improved high-protein breeding lines from northeastern USA. The breeding lines will be advanced and tested against appropriate checks. High performing lines will be released as germplasm or cultivars with the target traits. Conventional and molecular assisted breeding will be used. NIR protocols will be used for amino acid profiling of advanced breeding lines and finished germplasm. 3.Progress Report This three year United Soybean Board project started in April 01, 2011. Twenty advanced breeding lines with >45% seed protein obtained from the just concluded three year project (2008-11) have been planted at four locations in Ohio for evaluation of protein as well as other agronomic traits, including yield. More than 10,000 F4 and F5 seeds obtained from Puerto Rico have been planted in Wooster for selection of single plants in the Fall. Ten more crosses were done during the summer of 2011. This research relates to objective 4 (Develop soybean germplasm with high-yield and high seed-protein content) of the parent project.