1a.Objectives (from AD-416):
Increase protein contents, improve amino acid profile, and decrease phytate in MG-II to IV by incorporating appropriate genes from diverse sources of soybean lines.
1b.Approach (from AD-416):
The objective will be achieved by making crosses with high-protein, low phytate sources from other state cooperators to elite soybean lines and improved high-protein breeding lines from northeastern USA. The breeding lines will be advanced and tested against appropriate checks. High performing lines will be released as germplasm or cultivars with the target traits. Conventional and molecular assisted breeding will be used. NIR protocols will be used for amino acid profiling of advanced breeding lines and finished germplasm.
Three hundred plant rows were selected from more than 2000 plants rows derived from 20 crosses of Ohio soybean cultivars with high-protein breeding lines from Georgia, Arkansas, Missouri, North Carolina, Minnesota, and Ontario in 2012. Several advanced lines with >45% seed protein and high-yield from Ohio x Georgia crosses were also tested for yield at multiple locations in Ohio in 2012. These lines are in the final stages of yield testing and are expected to be released as germplasm by 2015. We are yield testing more than 300 selected plant rows in 2013 in replicated yield trials. The amino acids on high protein-lines were determined by Near Infra Red (NIR) technology using ground soybean samples. The increase in protein contents was not correlated to reduction in sulfur containing amino acids (e.g., Cysteine and methionine).
This project relates to and expands on objective 4 (Develop soybean germplasm with high seed-protein content) of the parent project.