1a.Objectives (from AD-416):
Produce large stock of vaccine virus for lyophilization and evaluation of the viability and effectiveness of vaccines in eggs and chickens.
1b.Approach (from AD-416):
1. Large scale of production of vaccine viruses and evaluation of titers (months 1-3).
2. Evaulation of stability of lyophilized tablets (months 4-9).
3. Evaluation of final formulation capacity to generate an immune response in chickens (months 7-9).
4. Final report (month 9).
This research is directly related to inhouse objective 3 - Develop vaccine strategies to effectively control Newcastle disease and stop disease outbreaks by developing vaccine platforms specifically designed to control low virulent and virulent Newcastle disease outbreaks.
The objectives were fully meet. An optimum formulation for lyophilized tablets was found and the effect of time and temperature on the viability of the vaccine was determined. Experiments in animals to confirm the usefulness of the tablet as vaccines were done and it was determined that the tablet formulated vaccine is as effective as the non encapsulated vaccine in protecting against virulent viruses. A publication is in progress.