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United States Department of Agriculture

Agricultural Research Service

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Research Project: ANALYSIS OF THE COLONIZATION OF CITRUS SEED COATS BY THE CAUSAL AGENT OF CITRUS HUANGLONGBING 'CA. LIBERIBACTER ASIATICUS'

Location: Subtropical Plant Pathology Research

Project Number: 6618-22000-039-17
Project Type: Reimbursable

Start Date: Jun 01, 2011
End Date: May 31, 2013

Objective:
Study colonization of seed coats by 'Ca. Liberibacter asiaticus'and devise methods of extracting the bacteria for research.

Approach:
We have identified seed coats of several citrus varieties as having high levels of 'Ca. Liberibacter asiaticus' DNA and in immature seeds and use microscopy to demonstrate viability of bacteria. We will determine if there are optimal stages of development of the seed for extraction of bacteria by sampling populations of seeds collected from fruit at various stages of development. We will test various mechanical methods to purify intact cells from the seed coats.

Last Modified: 9/22/2014
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