1a.Objectives (from AD-416):
The objectives of this project will be to: . 1)promote joint research and disseminate the experience of evaluating genetically engineered resistance to plum pox virus in Europe; and. 2)demonstrate the effectiveness, stability, and safety of the genetically engineered plum variety, 'HoneySweet', in Europe.
1b.Approach (from AD-416):
Scientific exchanges will be undertaken in order to collaborate on research projects and field tests of 'HoneySweet' plum. European researchers will work in the U.S. with ARS scientists in molecular characterization of gene expression in 'HoneySweet' trees and fruit. U.S. researchers will work in Europe with the evaluation and data collection from Europe field tests of 'HoneySweet' plum.
Work was completed on analyzing the presense of specific small RNAs in ‘HoneySweet’ fruit at different ripening stages and leaf material from trees unexposed to Plum Pox Virus. Additionally, fruit and leaf material from ‘HoneySweet’ trees grown in Europe was also analyzed through RNA blots for the presence of small RNAs at specific regions of the coat protein transgene.