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United States Department of Agriculture

Agricultural Research Service

Research Project: A Diagnostic Toolbox for Management of Apple Postharvest Necrotic Disorders - Agrofresh

Location: Plant, Soil and Nutrition Research

Project Number: 8062-21000-037-04
Project Type: Trust

Start Date: Nov 01, 2010
End Date: Sep 29, 2014

Objective:
To identify gene expression patterns specific to individual disorders which can be developed into biomarkers for predicting apple fruit disorders, thus determining storage plans for fruit held in controlled atmosphere storage.

Approach:
Facilitate RNAseq transcriptome analysis of apple tissues under conditions conducive to apple fruit postharvest disorders. We will harvest apple tissues, extract mRNA, create primer-tagged cDNA libraries, sequence said libraries via Illumina HighSeq, sort and trim sequences and process for quality, map to the public apple genome sequence and determine expression patterns via read counting. Resulting data will then feed into the larger NIFA funded apple consortium project for selection and development of biomarkers.

Last Modified: 10/20/2014
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