2012 Annual Report
1a.Objectives (from AD-416):
1) Update calibrations for grain composition and amino acids,.
2)update calibrations for and standardize five cooperator instruments, and.
3)train and support cooperators in quality control and lab operations.
1b.Approach (from AD-416):
The calibration samples will be provided by collaborating plant breeders. We estimate that 400 total samples per year will be screened for possible use as calibration samples. The GCL NIRS values will be compared to any original values from the breeder cooperator, which will provide an estimate of testing variability (standard deviation). GQL will scan the samples, and will collect the spectral data, sample information, and measured values. Samples for reference analysis (n=100; 50, 50, 50 per year, respectively) will be sent to the University of Missouri. Calibrations will be validated annually according to AACC Method 39-00, and updated if needed by April 30 of the respective marketing year. Updates are done by inclusion of the new samples in the calibration data set of the machine brand being evaluated. Calibrations/instrument setup for the following fall harvest will be done by the start of harvest. Temperature stability is introduced/verified by testing the 10-annual temperature samples at four temperatures (-5, 5, 25, and 45oC), using the accuracy criteria of AACC Method 39-00 to determine if additional temperature stability is needed. Standardization/traceability to the source of calibrations is necessary for accurate data from users’ instruments. Five cooperator instruments will be standardized against the calibration master units at GQL and against the wet chemistry references from University of Missouri, using 40 corn samples that have at least 3 replicate reference values for each factor. Annual verification of the standardization will be done with data from the cooperator samples. A new standardization with the GQL master samples will be needed if the calibrations are changed during the project period. The presence of these verified cooperator units will form the basis for a network of testing sites for cooperators specializing in marketing or utilizing the kinds of organic grains we are breeding. Project cooperators will determine the ongoing operating structure of this network, if created. Initial training, then annual support will be held with cooperators (at the time of the annual project meeting), with the intent to have cooperators self-sufficient in quality control and lab operations before the end of the project.
Iowa State University (ISU) continued to improve their Near Infrared (NIR) calibrations for the essential amino acids lysine, methionine and cysteine and protein, oil and starch by sending poorly predicted samples to the Experiment Station Chemistry Laboratory at the University of Missouri for laboratory analysis. The resulting data were used to develop new calibration equations. These calibration equations were provided to ARS and installed on the ARS Foss 1241.