2012 Annual Report 1a.Objectives (from AD-416): Develop and release new MG4-6 conventional and RR breeding lines with high protein, high sucrose, low stachyose, low phytate, and high yield potential as variety or germplasm. 1b.Approach (from AD-416): Develop and test, using conventional breeding methodology, a large number of lines and breeding populations for combining high protein, high digestible sugars, low oligosaccharides, and low phytate in the elite background. Our goal is for the yield level of the best high protein and low phytate lines to be comparable to popular commercial varieties. We will also develop a number of lines with low phytate and/or low stachyose without seed germination problems. 3.Progress Report: This project is related to Objective 2 of the in-house project: To characterize the biological mechanisms that control phytic acid concentration in soybean seed, and determine the impact of genetically lowering phytic acid on seed and seedling vigor, soy protein functionality and soybean meal digestibility. R05-1415 and R05-1772 were developed and released from the University of Arkansas Experiment Station, Fayetteville, AR in 2010. On average, R05-1415 yielded 94% of the high-yielding MG V conventional cultivar 5002T and had a protein content of 46.9%. Average yield of R05-1772 was 91% of 5002T with a protein content of 46.1%. Both R05-1415 and R05-1772 will be excellent parents for high protein soybean breeding. Sucrose and phosphorus content for R07-2001 was 8.2% and 1094.5 ug/g, respectively. R09-4606 had 5.5% of sucrose and 489.8 ug/g of phosphorus. R07-2004 and R07-2003 yielded 86% of check yield average (56.1bu/ac), with sucrose content of 8.8 and 6.9%, and the phosphorus content of 946.9 and 1253.0 ug/g, respectively.