2012 Annual Report 1a.Objectives (from AD-416): Develop and release maturity group (MG) 00, 0 and I lines with 1-3% higher protein and competitive yield; MG 00, 0, I lines with 3-6% higher protein, higher protein lines with hazard resistance, MG 00, 0, I lines with lower phytate and 1-3% higher protein, MG 00, 0, I lines with higher MET, CYS, THR, LYS. 1b.Approach (from AD-416): Cross higher protein lines with higher yielding lines, advance populations via in-breeding, select plants/lines with higher protein and/or lower phytate, yield test selected lines. 3.Progress Report: This project is related to Objective 2 of the in-house project: To discover novel genes/alleles in soybean for seed composition, determine their inheritance, determine genomic location, transfer to adapted germplasm, and release. The data from all the 2011 plots for higher protein and/or lower phytate have been analyzed. Selections for advancement have been made for the protein lines, but we are currently analyzing lines for phytate content. One line, M02-495076, that has been in the Regional Quality Tests with higher protein has been released for commercial use. Phytate lines that are being considered for advancement include 5 lines with lower phytate and 6 lines with low phytate and soybean cyst nematode (SCN) resistance. The average yield of these lines is about 107% of the mean with a range from 102-115%. Two lines are suggested for use by others in crossing. They are: M04-377006, a maturity group 0 line with low phytate and about 38% protein and M04-372-14404, a maturity group I line with low phytate and about 38% protein.