High Throughput SNP Genotyping in Chickpea Minicore Collection
Plant Germplasm Introduction and Testing
2011 Annual Report
1a.Objectives (from AD-416)
Development of chickpea genomic resources for discovering genes controlling economic traits, via association mapping of germplasm accessions.
1b.Approach (from AD-416)
The project will genotype a representative chickpea minicore collection of 200 germplasm lines, and 90 wild crop relatives of chickpea we have assembled at Pullman, with a 768 SNP (single nucleotide polymorphism) genotyping assay developed by UCD and ICRISAT, according to published criteria and in consultation with the ADOR. Illumina GoldenGate based genotyping developed by UCD and ICRISAT and the collaborative genotyping represents substantial savings over a stand alone project. This collaboration will allow ARS Pullman to gain genomics technologies, foster integration into an international consortium in chickpea genomics, and identify specific follow on research avenues of interest to chickpea breeding in the Pacific Northwest production area.
Progress was made to objective 1 from parent project. Seeds samples are ready for shipping to the collaborators and the genotyping team is designing the primers for the 768 SNP (single nucleotide polymorphism) markers for the project. Monitoring of progress was made through conference calls and emails.