2012 Annual Report 1a.Objectives (from AD-416): (1) Survey and collection of natural enemies (parasitoids) associated with emerald ash borers and buprestids infesting ash (Fraxinus spp) trees; (2) Import natural enemies to U.S. for quarantine evaluation; and (3) Investigate the role of natural enemies in regulating the pest populations. 1b.Approach (from AD-416): Investigate natural enemies of emerald ash borers on girdled ash trees in Russia at several suitable study sites determined from the previous study. Inventory and collect natural enemies attacking the target pest, assess role in population dynamics of pest, set priorities for importation, and ship promising species to Newark for further study. Monitor and sample selected sites intensively to determine the role of natural enemies in population dynamics. Hand carry or ship by air freight, natural enemies to U.S. 3.Progress Report: Chinese collaborator surveyed ash trees (Fraxinus mandchurica) in a Botanical Garden near Harbin City and Yilin Forest Farm of Yichun City (approximately 330 Km from Harbin) for occurrence of the emerald ash borer and associated egg parasitoids. In addition, 12 Manchurian ash trees were girdled in May of 2012 to attract emerald ash borers and associated natural enemies. Those trees will be sampled for both egg and larval parasitoids in the fall of 2013.