2011 Annual Report
2. Detect and characterize foodborne pathogens, toxins, and bacterial threat agents with rapid optical methods.
3. Develop and evaluate detection methods for foodborne pathogens and toxins with nanotechnology.
Acousto-Optic Tunable Filter Hyperspectral Microscope for Foodborne Pathogenic Bacteria Detection. A hyperspectral microscope imaging system could be an effective tool to understand the optical properties of foodborne pathogenic bacteria at the cell level. A hyperspectral microscope imaging method was developed for image acquisition with a dark field illumination light source. Salmonella, E.coli and biofilms were tested and their spectral characteristics were analyzed to identify spectral characteristics of different bacteria. Further development of this technique could be used for in-situ foodborne pathogen detection.
Rapid Detection of Salmonella using Surface Enhanced Raman Spectroscopy (SERS) with Silver Nanorods. SERS with silver nanosubstrate deposited on thin titanium coated glass slides can improve sensitivity for pathogen detection. Salmonella typhimurium were tested in the forms of live and dead cells. Spectra from the cells on the silver nanosubstrate were acquired with a confocal Raman microscope. Spectral signatures of the dead and live cells were compared to differentiate them. The spectral signatures from each cell type indicated structural changes in bacteria cell components. Thus, the SERS method could differentiate between live and dead bacteria cells.
Berrang, M.E., Windham, W.R., Meinersmann, R.J. 2011. Campylobacter, Salmonella and Escherichia coli on broiler carcasses subject to a high pH scald and low pH postpick chlorine dip. Poultry Science. 90(4):896-900.
Park, B., Yoon, S.C., Windham, W.R., Lawrence, K.C., Heitschmidt, G.W., Kim, M.S., Chao, K. 2011. Line-scan hyperspectral imaging for real-time on-line poultry fecal detection. Sensing and Instrumentation for Food Quality and Safety. 5:25-32.
Ko, S., Kim, J., Park, B., Cho, Y. 2011. Nanotechnology in food quality and safety evaluation systems. Emerging Technologies for Food Quality and Food Safety Inspection. p. 376.
Park, B., Lawrence, K.C., Windham, W.R. 2010. Imaging: Hyperspectral Contaminant Detection. In: Heldman, D.R., Moraru, C.I., editors. Encyclopedia of Agricultural, Food, and Biological Engineering. 2nd edition. Boca Raton, FL:Taylor & Francis. p. 854-857.
Park, B., Yoon, S.C., Windham, W.R., Lawrence, K.C. 2011. In-plant test of in-line multispectral imaging system for fecal detection during poultry processing. Applied Engineering in Agriculture. 27(4):623-630.
Samuel, D.D., Park, B., Sohn, M., Wicker, L. 2011. Visible/near-infrared spectroscopy to predict pale broiler breast meat by measuring water holding capacity. Poultry Science. 90:914-921.