1a.Objectives (from AD-416)
Identify the molecular mechanisms that underlie the interaction of enteric pathogens with fresh-cut leafy vegetables.
1b.Approach (from AD-416)
Transcriptomics in Salmonella and pathogenic E. coli; bacterial population dynamics on fresh-cut lettuce and spinach; and use of lettuce lines and Arabidopsis mutants that are defective in specific basal defense signaling pathways.
Studies under this project at the USDA have not fully begun yet because a technician to carry out the studies has not been hired due to a government freeze on hiring in the spring. The freeze has now been lifted. However, samples of shredded inoculated and uninoculated Romaine lettuce incubated for various time periods have been prepared and plant RNA was extracted for quantitative polymerase chain reacions (QRT-PCR). The PI at the Illinois Institute of Technology has constructed mutants of Salmonella in order to test for survival on processed lettuce. The Pinciple Investigator(PI) at IIT and the Co-PIs at ARS have been communicating via email.