2012 Annual Report 1a.Objectives (from AD-416): The purpose of this reimbursable cooperative agreement is to analyze corn samples for mycotoxin especially aflatoxin formulation trials for two strains of non-toxigenic Aspergillus flavus (A. flavus), K 49 & NRRL 21882. 1b.Approach (from AD-416): Several Corn Samples will be delivered by Syngenta and tested in an ARS laboratory using HPLC or LC/MS method. The following procedures and tasks will be carried out by USDA and Syngenta personnel: Syngenta will deliver to Investigator one combined sample per plot. ARS Investigator will grind, subsample and determine Aflatoxin levels for four major strains. 3.Progress Report: Field corn samples (total 380 samples) were received in fall of 2011 and analyzed for aflatoxins and some were analyzed for (CPA) by High-performance liquid chromatograhy (HPLC) and liquid chromatogray mass spectrometry (LC/MS). Fungal isolation of Aspergillus flavus strains were isolated from several samples. All data and results were reported to Syngenta. Monitoring of this project continues through frequent visits, telephone and e-mail communications.