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United States Department of Agriculture

Agricultural Research Service

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Research Project: Analyses of Corn Samples for Mycotoxins

Location: Biological Control of Pests Research Unit

2013 Annual Report

1a.Objectives (from AD-416):
The purpose of this reimbursable cooperative agreement is to analyze corn samples for mycotoxin especially aflatoxin formulation trials for two strains of non-toxigenic Aspergillus flavus (A. flavus), K 49 & NRRL 21882.

1b.Approach (from AD-416):
Several Corn Samples will be delivered by Syngenta and tested in an ARS laboratory using HPLC or LC/MS method. The following procedures and tasks will be carried out by USDA and Syngenta personnel: Syngenta will deliver to Investigator one combined sample per plot. ARS Investigator will grind, subsample and determine Aflatoxin levels for four major strains.

3.Progress Report:

Field corn samples (total 310 samples) were received in fall of 2012 and analyzed for Aflatoxins, fumonisin, and Cyclopiazonic Acid (CPA) by High Performance Liquid Chromatography (HPLC), enzyme-linked immunosorbent assay (ELISA) and Liquid Chromatography/ Mass Spectrometry (LC/MS). Fungal isolation of Aspergillus flavus strains were isolated from several samples. All data and results were reported to the cooperator. Monitoring of this project continues through frequent visits, telephone and e-mail communications.

Last Modified: 9/2/2015
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