1a.Objectives (from AD-416):
The purpose of this reimbursable cooperative agreement is to analyze corn samples for mycotoxin especially aflatoxin formulation trials for two strains of non-toxigenic Aspergillus flavus (A. flavus), K 49 & NRRL 21882.
1b.Approach (from AD-416):
Several Corn Samples will be delivered by Syngenta and tested in an ARS laboratory using HPLC or LC/MS method. The following procedures and tasks will be carried out by USDA and Syngenta personnel: Syngenta will deliver to Investigator one combined sample per plot. ARS Investigator will grind, subsample and determine Aflatoxin levels for four major strains.
Field corn samples (total 310 samples) were received in fall of 2012 and analyzed for Aflatoxins, fumonisin, and Cyclopiazonic Acid (CPA) by High Performance Liquid Chromatography (HPLC), enzyme-linked immunosorbent assay (ELISA) and Liquid Chromatography/ Mass Spectrometry (LC/MS). Fungal isolation of Aspergillus flavus strains were isolated from several samples. All data and results were reported to the cooperator. Monitoring of this project continues through frequent visits, telephone and e-mail communications.