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United States Department of Agriculture

Agricultural Research Service

Research Project: Technologies for Detecting and Determining the Bioavailability of Bacterial Toxins

Location: Foodborne Toxin Detection and Prevention

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)

New high-affinity monoclonal antibodies against Shiga toxin 1 facilitate the detection of hybrid Stx1/Stx2 in vivo - (Peer Reviewed Journal)
Skinner, C.B., Patfield, S.A., Stanker, L.H., Fratamico, P.M., He, X. 2014. New high-affinity monoclonal antibodies against Shiga toxin 1 facilitate the detection of hybrid Stx1/Stx2 in vivo. PLoS One. 9(6):e99854. DOI:10.1371journal.pone0099854.
Quantitative analysis of Staphylococcus enterotoxin A by differential expression of IFN-gamma in splenocyte and CD4+ T-cells - (Peer Reviewed Journal)
Rasooly, R., Hernlem, B.J. 2014. Quantitative analysis of Staphylococcus enterotoxin A by differential expression of IFN-gamma in splenocyte and CD4+ T-cells. Sensors. 14:8869-8876 DOI:10.3390/s140508869.
Risk of ricin from commercial castor production in North America - (Book / Chapter)
Auld, D.L., Trostle, C.L., Miller, T.D., He, X., Duncan, R.W. 2014. Risk of ricin from commercial castor production in North America. Cherwonogrodky, J.W., editor. Ricin Toxin. Oak Park, Illinois:Bentham Science. p. 86-97.
Current technologies for detection of ricin in different matrices - (Book / Chapter)
He, X. 2014. Current technologies for detection of ricin in different matrices.Cherwonogrodky, J.W., editor. Ricin Toxin. Oak Park, Illinois:Bentham Science. p. 38-56.
Electron-beam is effective in reducing Escherichia coli on blueberries and extending the shelf life of the fruits - (Peer Reviewed Journal)
Kong, Q., Wu, A., Qi, W., Qi, R., Carter, J.M., Rasooly, R., He, X. 2014. Electron-beam is effective in reducing Escherichia coli on blueberries and extending the shelf life of the fruits. Postharvest Biology and Technology. 95(2014):28-35.
Microwave heating inactivates Shiga Toxin (Stx2) in pH 7.4 buffer and in reconstituted fat-free Milk and adversely affects the nutritional value of cell culture medium - (Peer Reviewed Journal)
Rasooly, R., Hernlem, B.J., He, X., Friedman, M. 2014. Microwave heating inactivates Shiga Toxin (Stx2) in pH 7.4 buffer and in reconstituted fat-free Milk and adversely affects the nutritional value of cell culture medium. Journal of Agricultural and Food Chemistry. 62:3301-3305 DOI.org/10.1021/jf500278a.
Antibody interactions with Ricinus communis agglutinins studied by biolayer interferometry - (Peer Reviewed Journal)
Brandon, D.L., Adams, L., Yang, L.L., Korn, A.M. 2014. Antibody interactions with Ricinus communis agglutinins studied by biolayer interferometry. Analytical Letters. 47(10):1747-1758. DOI:10.1080/00032719.2014.886693.
Atoxic derivative of botulinum neurotoxin A as a prototype vehicle for targeted delivery to neuronal cytoplasm - (Peer Reviewed Journal)
Vazques-Cintron, E.J., Vakulenko, M., Band, P.A., Stanker, L.H., Johnson, E.A., Ichtchenko, K. 2014. Atoxic derivative of botulinum neurotoxin A as a prototype vehicle for targeted delivery to neuronal cytoplasm. PLoS One. 9(1):E85517. DOI: 10.1371/JOURNAL.PONE.0085517.
A monoclonal antibody based capture ELISA for botulinum neurotoxin serotype B: toxin detection in food - (Peer Reviewed Journal)
Stanker, L.H., Scotcher, M.C., Cheng, L.W., Ching, K., McGarvey, J.A., Hodge, D., Hnasko, R.M. 2013. A monoclonal antibody based capture ELISA for botulinum neurotoxin serotype B: toxin detection in food. Toxins. 5(11):2212-2226. DOI:10.3390/toxins5112212.
Mouse in vivo neutralization of Escherichia coli Shiga toxin 2 with monoclonal antibodies - (Peer Reviewed Journal)
Cheng, L.W., Henderson II, T.D., Patfield, S.A., Stanker, L.H., He, X. 2013. Mouse in vivo neutralization of Escherichia coli Shiga toxin 2 with monoclonal antibodies. Infection and Immunity. 5:1845-1858. DOI: 10.3390/toxins5101845.
Development of monoclonal antibodies and immunoassays for sensitive and specific detection of Shiga Toxin Stx2f - (Peer Reviewed Journal)
Skinner, C.B., Patfield, S.A., Stanker, L.H., He, X. 2013. Development of monoclonal antibodies and immunoassays for sensitive and specific detection of Shiga Toxin Stx2f. PLoS One. 8(9):e76563 DOI: 10.1371/journal/pone.0076563.
A polyclonal antibody based immunoassay detects seven subtypes of Shiga toxin 2 produced by escherichia coli in human and environmental samples - (Peer Reviewed Journal)
He, X., Patfield, S.A., Hnasko, R.M., Rasooly, R., Mandrell, R.E. 2013. A polyclonal antibody based immunoassay detects seven subtypes of Shiga toxin 2 produced by escherichia coli in human and environmental samples. PLoS One. 8(10):e76368. DOI:10.1371/journal.pone.0076368.
Prediction of B-cell epitopes in Listeriolysin O, a cholesterol-dependent cytolysin secreted by Listeria monocytogenes - (Peer Reviewed Journal)
Jones, M.S., Carter, J.M. 2014. Prediction of B-cell epitopes in Listeriolysin O, a cholesterol-dependent cytolysin secreted by Listeria monocytogenes. Advances in Bioinformatics. DOI: org/10.1155/2014/871676.
Non-linear relationships between aflatoxin B1 levels and the biological response of monkey kidney vero cells - (Peer Reviewed Journal)
Rasooly, R., Hernlem, B.J., He, X. 2013. Non-linear relationships between aflatoxin B1 levels and the biological response of monkey kidney vero cells. Toxins. 5:1447-1461. doi:10.3390/toxins5081447.
Comprehensive laboratory evaluation of a highly specific lateral flow assay for the presumptive identification of ricin in suspicious white powders and environmental samples - (Peer Reviewed Journal)
Hodge, D.R., Prentice, K.W., Ramage, J.G., Prezioso, S., Gauthier, C., Swanson, T., Hastings, R., Basavanna, U., Datta, S., Sharma, S.K., Garber, E.A., Staab, A., Pettit, D., Drumgoole, R., Swaney, E., Estacio, P.I., Elder, I.A., Kovacs, G., Morse, B.S., Kellogg, R.B., Stanker, L.H., Morse, S.A., Pillai, S.P. 2013. Comprehensive laboratory evaluation of a highly specific lateral flow assay for the presumptive identification of ricin in suspicious white powders and environmental samples. Biosecurity and Bioterrorism. 4:237-250. DOI: 10.1089/bsp.2013.0053.
Structure of a bimodular botulinum neurotoxin complex provides insights into its oral toxicity - (Peer Reviewed Journal)
Lee, K., Gu, S., Jin, L., Le, T., Cheng, L.W., Strotmeier, J., Krue, A.M., Perry, K., Yao, G., Rummel, A., Jin, R. 2013. Structure of a bimodular botulinum neurotoxin complex provides insights into its oral toxicity. PLoS Pathogens. 9(10):e1003690. DOI: 10.1371/journal.ppat.1003690.
Low levels of aflatoxin B1, ricin and milk enhance recombinant protein production in mammalian cells - (Peer Reviewed Journal)
Rasooly, R., Hernlem, B.J., Friedman, M. 2013. Low levels of aflatoxin B1, ricin and milk enhance recombinant protein production in mammalian cells. PLoS One. 8(8):e71682. doi:10.1371/journal.pone.0071682.
Thousand-fold fluorescent signal amplification for mHealth diagnostics - (Peer Reviewed Journal)
(26-Jun-13)
Substrates and controls for the quantitative detection of active botulinum neurotoxin in protease-containing samples - (Peer Reviewed Journal)
Bagramyan, K., Kaplan, B.E., Cheng, L.W., Rummel, A., Kalkum, M. 2013. Substrates and controls for the quantitative detection of active botulinum neurotoxin in protease-containing samples. Analytical Chemistry. 85(11):5569-76. doi: 10.1021/ac4008418.
Purification and characterization of neurotoxin complex from a dual toxin gene containing Clostridium botulinum strain PS-5 - (Peer Reviewed Journal)
Singh, A.K., Sachdeva, A., Degrasse, J.A., Croley, T.R., Stanker, L.H., Hodge, D., Sharma, S.K. 2013. Purification and characterization of neurotoxin complex from a dual toxin gene containing Clostridium botulinum strain PS-5. The Protein Journal. 32(4):288-296. DOI: 10.1007/S10930-013-9486-1.
Development of an automated multiplexed immunomagnetic separation system for isolating Shiga toxin-producing Escherichia coli - (Abstract Only)
(20-Mar-13)
Translocation of botulinum neurotoxins and associated proteins across intestinal epithelial cells(Abstract) - (Abstract Only)
(16-Mar-13)
Purification and characterization of Shiga toxin 2f, an immunologically unrelated subtype of Shiga toxin 2 - (Peer Reviewed Journal)
Skinner, C.B., Mcmahon, S.A., Rasooly, R., Carter, J.M., He, X. 2013. Purification and characterization of Shiga toxin 2f, an immunologically unrelated subtype of Shiga toxin 2. PLoS One. 8(3):e59760. doi:10.1371/journal.pone.0059760.
Detection of botulinum neurotoxin serotypes A and B using a chemiluminescent versus electrochemiluminescent immunoassay in food and serum - (Peer Reviewed Journal)
Cheng, L.W., Stanker, L.H. 2013. Detection of botulinum neurotoxin serotypes A and B using a chemiluminescent versus electrochemiluminescent immunoassay in food and serum. Journal of Agricultural and Food Chemistry. 61(3):755-760 doi:10.1021/jf3041963.
Development and characterization of monoclonal antibodies against Shiga toxin 2 and their application for toxin detection in milk - (Peer Reviewed Journal)
He, X., Mcmahon, S.A., Skinner, C.B., Merrill, P.A., Scotcher, M., Stanker, L.H. 2013. Development and characterization of monoclonal antibodies against Shiga toxin 2 and their application for toxin detection in milk. Journal of Immunological Methods. 389(1-2):18-28. doi:10.1016/j.jim.2012.12.005.
A 7-plex microbead-based immunoassay for serotyping Shiga toxin-producing Escherichia coli - (Peer Reviewed Journal)
Clotilde, L.M., Bernard IV, C., Salvador, A., Lin, A., Lauzon, C., Muldoon, M., Xu, Y., Lindpaintner, K., Carter, J.M. 2012. A 7-plex microbead-based immunoassay for serotyping Shiga toxin-producing Escherichia coli. Journal of Microbiological Methods. 92(2):226-230. doi:10.1016/j.mimet.2012.11.023.
Immunosorbent analysis of ricin contamination in milk using colorimetric, chemiluminescence, and electrochemiluminescence detection - (Peer Reviewed Journal)
Brandon, D.L., Korn, A.M., Yang, L. 2013. Immunosorbent analysis of ricin contamination in milk using colorimetric,chemiluminescence, and electrochemiluminescence detection. Food and Agricultural Immunology. doi:10.1080/09540105.2012.753515.
Development and characterization of six monoclonal antibodies to hemagglutinin-70 (HA70) of Clostridium botulinum and their application in a sandwich ELISA - (Peer Reviewed Journal)
Scotcher, M.C., Cheng, L.W., Ching, K.H., Mcgarvey, J.A., Hnasko, R.M., Stanker, L.H. 2013. Development and characterization of six monoclonal antibodies to hemagglutinin-70 (HA70) of Clostridium botulinum and their application in a sandwich ELISA. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. 32(1):6-15. doi: 10.1089/mab.2012.0071.
Monoclonal antibodies and reagents for botulinum research - (Proceedings)
Stanker, L.H., Cheng, L.W. 2012. Monoclonal antibodies and reagents for botulinum research. The Botulinum Journal. 2(2):150-155 doi: 10.1504/tbj2012.050197.
Detection of botulinum neurotoxin serotype A, B, and F proteolytic activity in complex matrices with picomolar to femtomolar sensitivity - (Peer Reviewed Journal)
Dunning, F., Rige, D.R., Piazza, T.M., Stanker, L.H., Zeytin, F.N., Ward, T.C. 2012. Detection of botulinum neurotoxin serotype A, B, and F proteolytic activity in complex matrices with picomolar to femtomolar sensitivity. Applied and Environmental Microbiology. 78(21):7687-7697. DOI.1128/AEM.01664-12.
Replacement of animals in assays for detection of toxins in food(Abstract) - (Abstract Only)
(10-Jul-12)
Detoxification of castor meal through reactive seed crushing - (Peer Reviewed Journal)
Dubois, J., Piccirilli, A., Magne, J., He, X. 2012. Detoxification of castor meal through reactive seed crushing. Industrial Crops and Products. 43(1):194-199.
Milk inhibits the biological activity of ricin - (Peer Reviewed Journal)
Rasooly, R., He, X., Friedman, M. 2012. Milk inhibits the biological activity of ricin. Journal of Biological Chemistry. 287(33):27924-27929. doi:10.1074/jbc.M112.362988.
A single-step purification and molecular characterization of functional Shiga toxin 2 variants from pathogenic Escherichia coli - (Peer Reviewed Journal)
He, X., Quinones, B., Mcmahon, S.A., Mandrell, R.E. 2012. A single-step purification and molecular characterization of functional Shiga toxin 2 variants from pathogenic Escherichia coli. Toxins. 4(7):487-504. doi:10.3390/toxins4070487
Botulinum neurotoxin: Where are we with detection technologies - (Peer Reviewed Journal)
Singh, A.K., Stanker, L.H., Sharma, S.K. 2013. Botulinum neurotoxin: Where are we with detection technologies. Critical Reviews in Microbiology. 39(1):43-56. doi:10.3109/1040841X.2012.691457.
TNF as biomarker for rapid quantification of active Staphylococcus enterotoxin A in food - (Peer Reviewed Journal)
Rasooly, R., Hernlem, B.J. 2012. TNF as biomarker for rapid quantification of active Staphylococcus enterotoxin A in food. Sensors. 12:5978-5985. doi:10.3390/s120505978.
Techniques for rapid detection and quantification of active foodborne Staphylococcus Enterotoxin(Abstract) - (Abstract Only)
Hernlem, B.J., Rasooly, R. 2012. Techniques for rapid detection and quantification of active foodborne Staphylococcus Enterotoxin(Abstract). Meeting Abstract. Poster no. B266.
Development of biphasic medium for detection of Shiga toxin producing E. coli using Tetrahymena thermophila - (Abstract Only)
(17-Feb-12)
Microbead based assays for detection and characterization of E. coli in pure cultures - (Abstract Only)
(17-Feb-12)
Detection and isolation of non-O157 STECs - (Abstract Only)
(17-Feb-12)
Sensitive bioassay for detection of biologically active ricin in food - (Peer Reviewed Journal)
Rasooly, R., He, X. 2012. Sensitive bioassay for detection of biologically active ricin in food. Journal of Food Protection. 75(5):951-954.
Detection of ricin contamination in liquid egg by electrochemiluminescence immunosorbent assay - (Peer Reviewed Journal)
Brandon, D.L., Korn, A.M., Yang, L. 2012. Detection of ricin contamination in liquid egg by electrochemiluminescence immunosorbent assay. Journal of Food Science. 77(4):T83-88.
Detection of botulinum neurotoxin serotypes A and B using chemiluminescence and electrochemiluninescene immunoassays in food and serum matrices - (Abstract Only)
(21-Dec-11)
Dynex: multiplex ELISA technology - (Peer Reviewed Journal)
Clotilde, L.M., Bernard Iv, C., Sequera, D.E., Karmali, A., Fusellier, A., Carter, J.M. 2012. Dynex: multiplex ELISA technology. Journal of Laboratory Automation. 17:309-314.2012.
Development of a faster method for detection of Shiga toxin producing E. coli using Tetrahymena thermophila - (Abstract Only)
(15-Oct-11)
Dynex: macrobead multiplex ELISA for STEC - (Abstract Only)
(15-Oct-11)
Electrochemiluminescence immunosorbent assay of ricin in ground beef: Biotinylated capture antibodies and matrix effects     Reprint Icon - (Peer Reviewed Journal)
Brandon, D.L. 2012. Electrochemiluminescence immunosorbent assay of ricin in ground beef: Biotinylated capture antibodies and matrix effects. Food and Agricultural Immunology. 23(4):329-337.
Neuronal targeting, internalization, and biological activity of a recombinant atoxic derivative of botulinum neurotoxin A - (Abstract Only)
(07-Oct-11)
Cd154 As a Potential Early Molecular Biomarker for Rapid Quantification Analysis of Active Staphylococcus Enterotoxin A - (Peer Reviewed Journal)
Rasooly, R., Hernlem, B.J. 2011. CD154 as a potential early molecular biomarker for rapid quantification analysis of active Staphylococcus enterotoxin A. FEMS Immunology and Medical Microbiology. 64(2):169-174.
Current methods for detecting the presence of botulinum neurotoxins in food and other biological samples - (Book / Chapter)
Cheng, L.W., Land, K.M., Stanker, L.H. 2012. Current methods for detecting the presence of botulinum neurotoxins in food and other biological samples. Morse, S., editor. Bioterrorism. Rijeka, Croatia: Intech. p. 1-16.
Evaluation and comparison of three enzyme-linked immunosorbent assay formats for the detection of ricin in milk and serum - (Peer Reviewed Journal)
He, X., Mcmahon, S.A., Rasooly, R. 2012. Evaluation and comparison of three enzyme-linked immunosorbent assay formats for the detection of ricin in milk and serum. Biocatalysis and Agricultural Biotechnology. 1(2):105-109. doi:10.1016/j.bcab.2011.08.016.
Food compounds inhibit Staphylococcus aureus bacteria and the toxicity of Staphylococcus Enterotoxin A (SEA) associated with atopic dermatitis - (Book / Chapter)
Rasooly, R., Friedman, M. 2012. Food compounds inhibit Staphylococcus aureus bacteria and the toxicity of Staphylococcus Enterotoxin A (SEA) associated with atopic dermatitis. In: Esparza-Gordillo, J., editor. Atopic Dermatitis-Disease Etiology and Clinical Management. Croatia: Intech Europe. p. 387-404
Rapid O serogroup identification of the ten most clinically relevant STECs by Luminex microbead-based suspension array - (Peer Reviewed Journal)
Lin, A., Nguyen, L., Lee, T., Clotilde, L.M., Kase, J.A., Son, I., Carter, J.M., Lauzon, C.R. 2011. Rapid O serogroup identification of the ten most clinically relevant STECs by Luminex microbead-based suspension array. Journal of Microbiological Methods. 87(1):105-110. doi:10.1016/j.mimet.2011.07.019.
Last Modified: 12/22/2014
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