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Agricultural Research Service United States Department of Agriculture
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Research Project: Technologies for Detecting and Determining the Bioavailability of Bacterial Toxins

Location: Foodborne Contaminants Research

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)

Translocation of botulinum neurotoxins and associated proteins across intestinal epithelial cells(Abstract) - (Abstract Only)
(16-Mar-13)
Purification and characterization of Shiga toxin 2f, an immunologically unrelated subtype of Shiga toxin 2 - (Peer Reviewed Journal)
Skinner, C.B., Mcmahon, S.A., Rasooly, R., Carter, J.M., He, X. 2013. Purification and characterization of Shiga toxin 2f, an immunologically unrelated subtype of Shiga toxin 2. PLoS One. 8(3):e59760. doi:10.1371/journal.pone.0059760.
Detection of botulinum neurotoxin serotypes A and B using a chemiluminescent versus electrochemiluminescent immunoassay in food and serum - (Peer Reviewed Journal)
Cheng, L.W., Stanker, L.H. 2013. Detection of botulinum neurotoxin serotypes A and B using a chemiluminescent versus electrochemiluminescent immunoassay in food and serum. Journal of Agricultural and Food Chemistry. 61(3):755-760 doi:10.1021/jf3041963.
Development and characterization of monoclonal antibodies against Shiga toxin 2 and their application for toxin detection in milk - (Peer Reviewed Journal)
He, X., Mcmahon, S.A., Skinner, C.B., Merrill, P.A., Scotcher, M., Stanker, L.H. 2013. Development and characterization of monoclonal antibodies against Shiga toxin 2 and their application for toxin detection in milk. Journal of Immunological Methods. 389(1-2):18-28. doi:10.1016/j.jim.2012.12.005.
A 7-plex microbead-based immunoassay for serotyping Shiga toxin-producing Escherichia coli - (Peer Reviewed Journal)
Clotilde, L.M., Bernard IV, C., Salvador, A., Lin, A., Lauzon, C., Muldoon, M., Xu, Y., Lindpaintner, K., Carter, J.M. 2012. A 7-plex microbead-based immunoassay for serotyping Shiga toxin-producing Escherichia coli. Journal of Microbiological Methods. 92(2):226-230. doi:10.1016/j.mimet.2012.11.023.
Immunosorbent analysis of ricin contamination in milk using colorimetric, chemiluminescence, and electrochemiluminescence detection - (Peer Reviewed Journal)
Brandon, D.L., Korn, A.M., Yang, L. 2013. Immunosorbent analysis of ricin contamination in milk using colorimetric,chemiluminescence, and electrochemiluminescence detection. Food and Agricultural Immunology. doi:10.1080/09540105.2012.753515.
Development and characterization of six monoclonal antibodies to hemagglutinin-70 (HA70) of Clostridium botulinum and their application in a sandwich ELISA - (Peer Reviewed Journal)
Scotcher, M.C., Cheng, L.W., Ching, K.H., Mcgarvey, J.A., Hnasko, R.M., Stanker, L.H. 2013. Development and characterization of six monoclonal antibodies to hemagglutinin-70 (HA70) of Clostridium botulinum and their application in a sandwich ELISA. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. 32(1):6-15. doi: 10.1089/mab.2012.0071.
Monoclonal antibodies and reagents for botulinum research - (Proceedings)
Stanker, L.H., Cheng, L.W. 2012. Monoclonal antibodies and reagents for botulinum research. The Botulinum Journal. 2(2):150-155 doi: 10.1504/tbj2012.050197.
Detoxification of castor meal through reactive seed crushing - (Peer Reviewed Journal)
Dubois, J., Piccirilli, A., Magne, J., He, X. 2012. Detoxification of castor meal through reactive seed crushing. Industrial Crops and Products. 43(1):194-199.
Milk inhibits the biological activity of ricin - (Peer Reviewed Journal)
Rasooly, R., He, X., Friedman, M. 2012. Milk inhibits the biological activity of ricin. Journal of Biological Chemistry. 287(33):27924-27929. doi:10.1074/jbc.M112.362988.
A single-step purification and molecular characterization of functional Shiga toxin 2 variants from pathogenic Escherichia coli - (Peer Reviewed Journal)
He, X., Quinones, B., Mcmahon, S.A., Mandrell, R.E. 2012. A single-step purification and molecular characterization of functional Shiga toxin 2 variants from pathogenic Escherichia coli. Toxins. 4(7):487-504. doi:10.3390/toxins4070487
Botulinum neurotoxin: Where are we with detection technologies - (Peer Reviewed Journal)
Singh, A.K., Stanker, L.H., Sharma, S.K. 2013. Botulinum neurotoxin: Where are we with detection technologies. Critical Reviews in Microbiology. 39(1):43-56. doi:10.3109/1040841X.2012.691457.
TNF as biomarker for rapid quantification of active Staphylococcus enterotoxin A in food - (Peer Reviewed Journal)
Rasooly, R., Hernlem, B.J. 2012. TNF as biomarker for rapid quantification of active Staphylococcus enterotoxin A in food. Sensors. 12:5978-5985. doi:10.3390/s120505978.
Techniques for rapid detection and quantification of active foodborne Staphylococcus Enterotoxin(Abstract) - (Abstract Only)
Hernlem, B.J., Rasooly, R. 2012. Techniques for rapid detection and quantification of active foodborne Staphylococcus Enterotoxin(Abstract). Meeting Abstract. Poster no. B266.
Sensitive bioassay for detection of biologically active ricin in food - (Peer Reviewed Journal)
Rasooly, R., He, X. 2012. Sensitive bioassay for detection of biologically active ricin in food. Journal of Food Protection. 75(5):951-954.
Detection of ricin contamination in liquid egg by electrochemiluminescence immunosorbent assay - (Peer Reviewed Journal)
Brandon, D.L., Korn, A.M., Yang, L. 2012. Detection of ricin contamination in liquid egg by electrochemiluminescence immunosorbent assay. Journal of Food Science. 77(4):T83-88.
Detection of botulinum neurotoxin serotypes A and B using chemiluminescence and electrochemiluninescene immunoassays in food and serum matrices - (Abstract Only)
(21-Dec-11)
Dynex: multiplex ELISA technology - (Peer Reviewed Journal)
Clotilde, L.M., Bernard Iv, C., Sequera, D.E., Karmali, A., Fusellier, A., Carter, J.M. 2012. Dynex: multiplex ELISA technology. Journal of Laboratory Automation. 17:309-314.2012.
Electrochemiluminescence immunosorbent assay of ricin in ground beef: Biotinylated capture antibodies and matrix effects     Reprint Icon - (Peer Reviewed Journal)
Brandon, D.L. 2012. Electrochemiluminescence immunosorbent assay of ricin in ground beef: Biotinylated capture antibodies and matrix effects. Food and Agricultural Immunology. 23(4):329-337.
Neuronal targeting, internalization, and biological activity of a recombinant atoxic derivative of botulinum neurotoxin A - (Abstract Only)
(07-Oct-11)
CD154 as a potential early molecular biomarker for rapid quantification analysis of active Staphylococcus enterotoxin A - (Peer Reviewed Journal)
Rasooly, R., Hernlem, B.J. 2011. CD154 as a potential early molecular biomarker for rapid quantification analysis of active Staphylococcus enterotoxin A. FEMS Immunology and Medical Microbiology. 64(2):169-174.
Current methods for detecting the presence of botulinum neurotoxins in food and other biological samples - (Book / Chapter)
Cheng, L.W., Land, K.M., Stanker, L.H. 2012. Current methods for detecting the presence of botulinum neurotoxins in food and other biological samples. Morse, S., editor. Bioterrorism. Rijeka, Croatia: Intech. p. 1-16.
Evaluation and comparison of three enzyme-linked immunosorbent assay formats for the detection of ricin in milk and serum - (Peer Reviewed Journal)
He, X., Mcmahon, S.A., Rasooly, R. 2012. Evaluation and comparison of three enzyme-linked immunosorbent assay formats for the detection of ricin in milk and serum. Biocatalysis and Agricultural Biotechnology. 1(2):105-109. doi:10.1016/j.bcab.2011.08.016.
Food compounds inhibit Staphylococcus aureus bacteria and the toxicity of Staphylococcus Enterotoxin A (SEA) associated with atopic dermatitis - (Book / Chapter)
Rasooly, R., Friedman, M. 2012. Food compounds inhibit Staphylococcus aureus bacteria and the toxicity of Staphylococcus Enterotoxin A (SEA) associated with atopic dermatitis. In: Esparza-Gordillo, J., editor. Atopic Dermatitis-Disease Etiology and Clinical Management. Croatia: Intech Europe. p. 387-404
Rapid O serogroup identification of the ten most clinically relevant STECs by Luminex microbead-based suspension array - (Peer Reviewed Journal)
Lin, A., Nguyen, L., Lee, T., Clotilde, L.M., Kase, J.A., Son, I., Carter, J.M., Lauzon, C.R. 2011. Rapid O serogroup identification of the ten most clinically relevant STECs by Luminex microbead-based suspension array. Journal of Microbiological Methods. 87(1):105-110. doi:10.1016/j.mimet.2011.07.019.

   

 
Project Team
Brandon, David
Carter, John - Mark
Cheng, Luisa Wai Wai
He, Xiaohua
Hernlem, Bradley - Brad
Rasooly, Reuven
Stanker, Larry
 
Project Annual Reports
  FY 2012
  FY 2011
 
Publications
   Publications
 
Related National Programs
  Food Safety, (animal and plant products) (108)
 
Related Projects
   CREATION AND PREPARATION OF MONOCLONAL ANTIBODIES FOR USE IN BIOLOGICAL TOXINS DETECTION ASSAYS
   ELECTROCHEMILUMINESCENT ASSAY FOR BOTULINUM NEUROTOXINS
   Anti-Botulism Monoclonal antibodies as tools to identify small molecule toxin inhibitors
   SIMULTANEOUS DETECTION OF MULTIPLE FOODBORNE PATHOGENS WITH A SINGLE ANTIBODY-BASED TEST
   DEVELOPMENT OF TECHNOLOGIES FOR DETECTION AND MITIGATION OF UNDESIRABLE ORGANISMS ASSOCIATED WITH FOOD
   DEVELOPMENT OF DETECTION TECHNOLOGIES FOR BACTERIAL NEUROTOXINS AND THEIR VALIDATION IN FOOD MATRICES
 
 
Last Modified: 05/23/2013
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