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United States Department of Agriculture

Agricultural Research Service

Related Topics

Research Project: Virus of Hemipterans: Leafhoppers and Psyllids

Location: Subtropical Insects and Horticulture Research

2013 Annual Report


1a.Objectives (from AD-416):
Evaluate virus infection as potential biological control agents against leafhoppers and psyllids, Homalodisca vitripennis and Diaphorina citri.


1b.Approach (from AD-416):
Infect leafhoppers and psyllids, by feeding or injections and monitor survival, female fecundity, and virus titers using PCR methods.


3.Progress Report:

This research relates to inhouse project objective 2: Biological Control: Develop new and improve existing biological control methods for psyllids and sharpshooter species. 2a. Virus propagation in insect cell cultures.

Leafhopper cell cultures from the glassy-winged sharpshooter, H. vitripennis, were used to evaluate costs associated with successful propagation of wild-type leafhopper-infecting viruses. The main road block to using natural pathogens for insect pest management is the ability to mass produce them in a cost efficient manner. The methods of virus production evaluated were not efficient enough to compete with synthetic pesticides; however, production levels were sufficient for expanding experimental trials towards using these viruses as delivery systems to suppress insect pests. Results were submitted for publication.


Last Modified: 11/23/2014
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