1a.Objectives (from AD-416)
Identify NSGC accessions with enhanced nitrogen and water use efficiency (NUE, WUE) and determine the genetic basis of the traits using association mapping.
1b.Approach (from AD-416)
Single-plant selections from the NSGC barley and wheat core collections will be grouped by maturity and screened in the field under varying nitrogen and water levels using an augmented experimental design. Canopy spectral radiometry and yield will be used to measure NUE and WUE. Experiments will be conducted over 5 cropping seasons. SNP data will be generated for the accessions as part of the Triticeae CAP project and data will be used to explore the genetic basis of enhanced NUE and WUE within the accessions.
The research contributes to the parent project objective 2 goal to strategically evaluate accessions from the collection for resistance to important abiotic stresses. This is a newly established agreement. The objective of this work is to identify accessions from the National Small Grains Collection that are drought resistance and have high nitrogen use efficiency. Currently, 545 lines are being assessed for coleoptile length, seedling root length, and root numbers in a lab germination test. Data will be used to correlate with data derived from field evaluations.
Progress of the project is monitored via meetings and site visits.