Developing Molecular Markers for Carya
Crop Germplasm Research
2013 Annual Report
1a.Objectives (from AD-416):
Characterize the genetic diversity of Carya using molecular genetic tools.
Develop new molecular genetic procedures as needed and appropriate. Use the information developed to guide the conservation of Carya populations and the selection and deployment of germplasm.
1b.Approach (from AD-416):
Microsatellite primers developed in previous research will be used to characterize accessions at the level of species, population, and individual, and to verify questions of parentage. New primers will be developed as needed. Other techniques, such as Amplified Fragment Length Polymorphisms (AFLPs) and Expressed Sequence Tag analysis (ESTs) will be pursued.
The goal of this project is to characterize the genetic diversity of Carya (pecan/hickory) using molecular genetic tools, develop new molecular genetic procedures as needed and appropriate, and use the information developed to guide the conservation of Carya populations and the selection and deployment of germplasm. Work during FY 2013 focused on the development of sequence based molecular markers in Carya. More than 2,500 molecular tools known as Single Nucleotide Polymorphisms (SNPs) were generated and used to establish the genetic relationships between nine Carya lines, demonstrating proof-of-concept for the techniques being applied. Limitations observed at several key steps will be addressed and refined as project work continues.