2013 Annual Report
1a.Objectives (from AD-416):
Extensively phenotype and genotype diverse and designed germplasm pools to mine and deploy alleles that improve barley and wheat adaptation and performance under biotic and abiotic stress, and couple these activities with the training of a new generation of plant breeders.
1b.Approach (from AD-416):
Develop and implement large- and small-scale SNP genotyping platforms for barley and wheat breeding programs, and test novel sequence-based genotyping platforms.
Integrate the genotypic and phenotypic information into GRIN, GrainGenes, and Gramene databases and develop web-based tools to help breeders use this information.
We will develop 48 and 384 SNP platforms dedicated to known genes or previously identified marker-trait associations for barley and wheat. These SNP platforms will be available at the Small Grains Genotyping Centers for breeders to characterize breeding lines in advanced yield trials. Simultaneously, we will test new genotyping technologies based on high-throughput sequencing platforms (e.g. gene capture and sequence-based genotyping) in barley and wheat and implement them as they become economically feasible.
This research relates directly to inhouse objective 2. Develop improved methods of marker-assisted selection, and apply markers in development of small grains cultivars.
Genotyping with 384 SNP was performed on winter barely lines included in the second and third cycle of a population undergoing genomic selection for improvement of low temperature tolerance. Genotyping with a panel of 384 SNP markers was also done on barley breeding lines to evaluate application of genomic selection for yield. KASP assays were developed and evaluated on a barley recombinant inbred line population to map resistance to Cereal Yellow Dwarf Virus.
Genomic DNA was isolated from individual plants and selfed seed was harvested from an elite panel of 288 winter wheat breeding lines genotyped with the new iSelect SNP assay having 90,000 wheat SNP.
Seventy-six winter wheat lines from the USDA National Small Grains Collection wheat core collection being used as parents for developing winter wheat nested association mapping population were genotyped with KASP assays for genes involved in plant development and disease resistance. Lines were evaluated in the greenhouse for vernalization requirement. Approximately 600 winter wheat accessions were evaluated in the field at Raleigh, NC for reaction to powdery mildew and flowering time.