Location: Meat Safety & Quality Research
2012 Annual Report
Objective 2. Develop and evaluate intervention strategies that reduce or eliminate the occurrence, persistence, or movement of foodborne pathogens among food animals, their environment, and potential surrounding production environments.
Developing effective strategies to reduce pathogens in cattle also will require the understanding of factors that affect pathogen occurrence and transmission in the production environment. There is increasing interest in the use of deep-bedded confinement facilities in the cattle feeding industry for a variety of reasons, including ease of manure management and improved cattle performance compared to open lot feedlots. In FY 2012, we completed studies to evaluate the influence of different bedding materials on the populations of E. coli in deep-bedded cattle waste, in order to identify bedding materials that may limit the growth and persistence of these bacteria. The work is part of a larger collaborative effort that is also seeking to quantify and characterize odor emissions from cattle deep-bedded barns, with the goal to develop recommendations for managing these facilities to reduce odor, gas emissions, and pathogens. In addition, we initiated studies to confirm a role for environmental persistence of E. coli O157:H7 in feedlot surface manure in the persistence and transmission of this pathogen in cattle. Airborne transport of E. coli O157:H7 is a potential mode of transmission of this pathogen among cattle in the production environment, as well as to the environments surrounding cattle production facilities. We continued studies to determine if E. coli O157:H7 can be transported in windborne bioaerosols from cattle feedlots.
In 2012, we examined production factors impacting pathogens in swine, as well as intervention strategies to reduce pathogens in swine. Studies were completed to determine if age of piglets at weaning affects pathogen shedding in swine fed antibiotic-free diets. In addition, studies with 21-day-old nursery piglets were completed, to determine the impacts of diets containing the natural antimicrobial lysozyme on production performance and fecal shedding of the pathogens Salmonella, Campylobacter, and/or Shiga-toxin producing E. coli, compared to diets with and without dietary antibiotic growth promoters.
Wells, J., Shackelford, S.D., Berry, E.D., Kalchayanand, N., Bosilevac, J.M., Wheeler, T.L. 2011. Impact of reducing the level of wet distillers grains fed to cattle prior to harvest on prevalence and levels of Escherichia coli O157:H7 in feces and on hides. Journal of Food Protection. 74(10):1611-1617. Berry, E.D., Wells, J. 2012. Soil solarization reduces Escherichia coli O157:H7 and total Escherichia coli on cattle feedlot pen surfaces. Journal of Food Protection. 75(1):7-13. Vogel, J.R., Gilley, J.E., Woodbury, B.L., Berry, E.D., Eigenberg, R.A. 2011. Transport of trace elements in runoff from unamended and pond-ash amended feedlot surfaces. Transactions of the ASABE. 54(4):1269-1279. Casas, E., Garcia, M.D., Wells, J., Smith, T.P.L. 2011. Association of single nucleotide polymorphisms in the ANKRA2 and CD180 genes with bovine respiratory disease and presence of Mycobacterium avium subsp. paratuberculosis. Animal Genetics. 42:571-577. May, K.D., Wells, J., Maxwell, C.V., Oliver, W.T. 2012. Granulated lysozyme as an alternative to antibiotics improves growth performance and small intestinal morphology of 10-day-old pigs. Journal of Animal Science. 90(4):1118-1125. Varel, V.H., Wells, J., Shelver, W.L., Rice, C., Armstrong, D.L., Parker, D.B. 2012. Effect of anaerobic digestion temperature on odour, coliforms and chlortetracycline in swine manure or monensin in cattle manure. Journal of Applied Microbiology. 112:705-715. Puchala, R., Animut, G., Patra, A.K., Detweiler, G.D., Wells, J., Varel, V.H., Sahlu, T., Goetsch, A.L. 2012. Methane emissions by goats consuming Sericea lespedeza at different feeding frequencies. Animal Feed Science And Technology. 175:76-84.