Start Date: Apr 03, 2010
End Date: Apr 02, 2015
Conduct laboratory and field experiments to examine the important biological, chemical, and physical processes affecting the prevalence and distribution of pathogenic and antibiotic resistant bacteria on representative farms in the Santa Ana River watershed. Studies will be conducted at various scales to determine the persistence (survival) of E. coli O157:H7 in its sources on these farms and assess potential factors influencing pathogen survival in the root zone and contamination of leafy greens. Laboratory scale study will be conducted to quantify critical processes influencing the dissemination of pathogens in the watershed by runoff, streams and rivers. Factors influencing the treatment of contaminated surface waters by sand filtration will also be investigated to more fully assess its capabilities and potential weaknesses. Data obtained from these studies will be used to develop best management practices (BMPs) and low cost treatment technologies for immobilization and inactivation of pathogens from concentrated animal feeding operations (CAFOs) to water and food resources.