1a.Objectives (from AD-416):
Develop immunologic tools to enhance our understanding of swine immune system development and host responses to mucosal diseases. The cooperator is interested in preventing infectious disease and controlling their spread through development of effective therapeutics and vaccines for cattle and swine.
1b.Approach (from AD-416):
Increase the understanding of how pigs responds to infection, by developing reagents that identify immune proteins [type I interferons (IFNs) and the IFN-alpha/beta receptor (IFNAR)] that regulate the early, or “innate” immune events. The Cooperator will assist in the development of similar reagents for cattle. Specifically, 19 reagents are identified by DHS and USDA-ARS as high priority reagents for US VIRN to develop to support foreign animal disease research programs (e.g., Foot and Mouth Disease; Rift Valley Fever; Classical Swine Fever).
New swine reagents were developed to help scientists probe immune responses to vaccines and infectious disease threats. ARS researchers at Beltsville Agricultural Research Center (BARC) in Beltsville, Maryland worked with Kansas State University and Cornell University scientists to express swine innate immune proteins, the interferons (IFNs), and used them to successfully develop monoclonal antibodies (mAbs). Separately the genes for important cell surface proteins, the IFNA receptor, natural killer (NK) and B cell markers were cloned and expressed. To date several panels of mAbs from Cornell have been screened at BARC and found to be reactive with their cell targets. Progress for these efforts has been documented on the U.S. Veterinary Immunological Reagents Network website (www.vetimm.org). Products generated will be used by animal health researchers, veterinarians, vaccine manufacturers, and other commercial sources to improve immune responses to infectious disease outbreaks develop better vaccines.