Development of a Non-Lethal Rapid Test for Determination of Intestinal Enteritis in Rainbow Trout
Small Grains and Potato Germplasm Research
2011 Annual Report
1a.Objectives (from AD-416)
Develop an in-vitro test to determine the presence of intestinal enteritis in rainbow trout.
1b.Approach (from AD-416)
Rainbow trout fingerlings (average 9 grams per fish initial weight) will be fed diets varying in type and level of soybean meal. After 16 weeks of feeding the experimental diets, blood from 40 fish in each tank will be sampled. Plamsa will be tested for LDH, bilirubin, immunoglobin, fibrinogen, ATPase. Plamsa will also be evaluated using NMR by EWOS Innovation in Norway.
This research is connected to the parent project through objective 3, “Determine nutritional value of alternative ingredients (protein, lipid, energy) and develop practical feed formulations for improved strains of fish”. Development of a rapid non-lethal test for determination of enteritis in trout will allow for a faster screening of potential feed ingredients for anti-nutrients. Feeding studies with standard and improved varieties of soybean meal have been conducted. Samples of intestines and other organs were taken for analysis and are being processed.
Communication and coordination was achieved with researchers from European Workshop for Open Systems (EWOS) Innovation and ARS visited the other laboratories and through frequent emails and phone conversations.