1a.Objectives (from AD-416):
Develop and optimize the effectiveness of bioremediation techniques for the abatement of odorous compounds in CAFO wastewater. Optimize the effectiveness of genetically modified yeast for abatement of odorous compounds. Evaluate a non-GMO based anaerobic bioreactor with complementary attributes.
1b.Approach (from AD-416):
A continuous flow bioreactor will be fabricated, optimized, and tested based upon genetically modified Saccharomyces cerevisiae (Baker’s yeast) displaying horseradish peroxidase (HRP). HPLC methods will be used for liquid phase quantification of phenolic and indolic odorants, and for CAFO manure lagoon and bioreactor performance optimization and evaluation. Effectiveness of the odor control and VOC remediation will be measured using GC/MS and thermal desorption tubes.
ARS scientists at Clay Center, Nebraska, conducted a lab-scale experiment to determine odor reduction in swine manure using the additives soybean peroxidase and calcium peroxide. The study was designed to determine best method of application, the amount of additives necessary for odor reduction and how long the additives reduced odors. Odors were collected by passing clean air over the top of the manure in small plastic buckets to represent deep-pit barn or swine lagoons.
All of the additive combinations and method of application were effective at reducing emissions between 0 and 7 d after application. However, after day 14 the odor emissions were higher for the treated manure indicating the treatment is effective for the first week after application, but that application may be necessary every 7 days.