1a.Objectives (from AD-416):
Develop and optimize the effectiveness of bioremediation techniques for the abatement of odorous compounds in CAFO wastewater. Optimize the effectiveness of genetically modified yeast for abatement of odorous compounds. Evaluate a non-GMO based anaerobic bioreactor with complementary attributes.
1b.Approach (from AD-416):
A continuous flow bioreactor will be fabricated, optimized, and tested based upon genetically modified Saccharomyces cerevisiae (Baker’s yeast) displaying horseradish peroxidase (HRP). HPLC methods will be used for liquid phase quantification of phenolic and indolic odorants, and for CAFO manure lagoon and bioreactor performance optimization and evaluation. Effectiveness of the odor control and VOC remediation will be measured using GC/MS and thermal desorption tubes.
As part of a National Research Initiative grant project, ARS scientists at Clay Center, Nebraska, conducted research to evaluate soybean-byproduct additives for reducing odorous volatile organic compounds from swine manure. A laboratory experiment was conducted to evaluate the effects of soybean peroxidase application rate and type of peroxide in a thoroughly mixed system. Odor compounds were quantified using previously developed methods in a small wind tunnel. A second experiment has been planned to evaluate topical application to swine manure.