Development of Rapid Detection Methods Using Nanobiosensors for Salmonella and Vaccine Against SE and ST for Poultry
Quality and Safety Assessment Research Unit
2012 Annual Report
1a.Objectives (from AD-416):
To develop a nanobiosensor to detect Salmonella using surface-enhanced Raman scattering (SERS) technique:.
1)determine SERS characteristics of Salmonella;.
2)develop chemometric models to differentiate Salmonella from other bacteria;.
3)classify different Salmonella strains; and.
4)evaluate detection limits of nanobiosensor.
1b.Approach (from AD-416):
We will develop methodologies to test and validate the SERS techniques. A series of experiments will be conducted based on the following methods:.
1)Design, fabricate SERS substrate to optimize for lower detection limits;.
2)Salmonella sample preparation;.
4)Test and validate classification models for different serotypes;.
4)Test detectability from different background flora.
This research relates to inhouse project objective 1. Develop methods and instruments to identify food safety hazards throughout various stages of poultry and egg production and processing.
The protocol for biopolymer encapsulated by silver nanoparticles was developed as surface enhanced Raman scattering (SERS) substrate. The characteristics of silver nano colloidal particle were investigated with morphological analysis, ultraviolet/visible plasma absorption analysis, and plasmonic resonance analysis to examine reproducibility and uniformity of the substrate. The plasmonic absorption peak between 400 and 480 nm was observed from the substrate. SERS with biopolymer silver nano-colloid substrate has potential to detect and classify Salmonella serotypes Typhimurium and Enteritidis.