Development of Rapid Detection Methods Using Nanobiosensors for Salmonella and Vaccine Against Se and St for Poultry
Quality and Safety Assessment Research Unit
Project Number: 6040-42000-041-03
Start Date: Oct 01, 2010
End Date: Sep 30, 2015
To develop a nanobiosensor to detect Salmonella using surface-enhanced Raman scattering (SERS) technique: (1) determine SERS characteristics of Salmonella; (2) develop chemometric models to differentiate Salmonella from other bacteria; (3) classify different Salmonella strains; and (4) evaluate detection limits of nanobiosensor.
We will develop methodologies to test and validate the SERS techniques. A series of experiments will be conducted based on the following methods: (1) design and fabricate SERS substrate to optimize for lower detection limits; (2) Salmonella sample preparation; (3) SERS measurement; (4) test and validate classification models for different serotypes; and (5) test detectability from different background flora.