PATHOGENESIS OF FOOT-AND-MOUTH DISEASE VIRUS STRAINS ISOLATED IN 2010 FROM THE REPUBLIC OF KOREA
Foreign Animal Disease Research
2013 Annual Report
1a.Objectives (from AD-416):
This cooperative research project focuses on the study of the pathogenicity of Foot-and-Mouth Disease Virus (FMDV) Korean isolates, A/ROK/2010 and O/ROK/2010 in pigs and cattle. Specific objectives are: 1. Conduct a time-course study of FMD infection in pigs and cattle; including direct transmission, 2. Obtain and catalog quality pictures of lesions and reference stocks of research material; serum and tissues.
1b.Approach (from AD-416):
NVRQS will provide FMDV A/ROK/2010 and O/ROK/2010 from pigs and cattle, as well as conduct epidemiological analysis and conduct genomic sequencing of viral isolates. ARS, PIADC will conduct FMDV pathogenicity studies on swine and cattle; inclusive of time-course study and histopathological evaluation and sample collection. NVRQS scientists will be trained to conduct research on FMDV pathogenesis using methology developed by ARS, PIADC scientists. Data generated will help to develop and upgrade current diagnostic assays for the detection of FMD circulating in south east Asia.
During FY 2012-13 Foot-and-Mouth Disease Virus (FMDV) O/Korea 2010 strain was amplified in swine and cattle to generate virus for future experiments. The cattle derived virus was successfully utilized to show cattle-to-cattle and cattle-to-swine transmission. During FY 2013 laboratory activities were completed and activities were focused on the transfer of the viruses from ARS, PIADC to NVRQS for deep sequence analysis which will be published. This research project is now undergoing administrative close-out.