Location: Plant, Soil and Nutrition Research
Project Number: 8062-21000-037-07
Start Date: Aug 01, 2011
End Date: Dec 31, 2015
Two complementary approaches will be used for the identification of the genes and proteins associated with Or in regulating carotenoid accumulation. A sensitive yeast two-hybrid will be employed to isolate the OR-interacting protein(s). As a systems approach provides a unique opportunity to identify components of metabolic pathways and map points of regulatory control, the systems approach that integrates transcriptome and bioinformatics tools will also be utilized to define the key genes and metabolic processes that impact carotenoid accumulation. Their involvement will be accessed genetically in melon and functionally confirmed in Arabidopsis callus system. The allelic variation in association with Beta-carotene content in melon will be examined in the germplasm collection to uncover the favorable alleles for breeding high Beta-carotene crops.