Location: Chemistry Research Unit
2013 Annual Report
1a. Isolation and identification of elicitors or biotic agents of induced plant volatile emission and other inducible plant defenses.
1b. Elucidation of the signaling interactions that mediate insect and insect elicitor induced plant defenses and volatile emission.
2. Identification and evaluation of chemicals that regulate or influence behaviors, including foraging, mating and oviposition, of important pest insects.
2a. Isolate and identify plant volatiles and insect produced pheromones that in combination attract male and female pepper and cranberry weevils.
2b. Identification of oviposition deterring pheromone from pepper weevil.
2c. Interaction between Insect and Host Volatiles on Reproductive Development of Anastrepha spp. (Dipera:Tephrididae).
2d. Identification of oviposition deterring pheromones for Tephritid Fruit Flies.
2e. Influence of Fruit Volatiles on attraction of Small Hive Beetle.
3. Improve the efficacy of alternatives to methyl bromide fumigation for management of stored-product insects by developing new attractants and behavior-altering chemicals that affect Sitophilous spp. (rice and maize weevils) and Tribolim castaneum (red flour beetle) for the purpose of understanding the chemical ecology of these insects and developing practical methods for detection and control.
Studies were conducted to gain mechanistic insight into how European corn borer may alter defense responses in maize tissues. Analysis of European corn borer oral secretions following ingestion of tryptophan demonstrated increased production rates of the plant phytohormone indole-3-acetic acid. Application of indole-3-acetic acid to maize stem tissues resulted in complete metabolism and removal within 12 hours and did not alter stem protein levels. Studies are on-going to supply continuous levels of indole-3-acetic acid as are present from larval excretions into the feeding tunnel.
The Pepper weevil oviposition deterring pheromone has been identified as a combination of two compounds with different functions.
Research on oviposition deterrent pheromones of Tephritid fruit flies of led to determination that oviposition deterrents deposited after oviposition have interspecific activity. Collection and separation of the deterrents by HPLC for two species has resulted in isolation of biologically active fractions which are currently being further purified.
Studies on molecular regulation of reproduction in the Varroa mite led to the complete sequencing of 5 genes responsible for reproductive competence. Physiological studies documented the effects of these genes and their interaction with honeybee development. The studies pave the way for development of molecular approaches to control the mite.
Choe, A., Chuman, T., Von Reuss, S.H., Doseey, A.T., Yim, J., Ajredini, R., Kolawa, A.A., Kaplan, F., Alborn, H.T., Teal, P.E., Schroeder, F.C., Sternberg, P.W., Edison, A.S. 2012. Sex-specific mating pheromones in the nematode Panagrellus redivivus. Proceedings of the National Academy of Sciences. 109(51):20949-20954.
Cabrera Cordon, A.R., Shirk, P.D., Duehl, A.J., Evans, J.D., Teal, P.E. 2013. Variable induction of vitellogenin genes in the varroa mite, Varroa destructor (Anderson & Trueman) by the honeybee, Apis mellifera L, host and its environment. Insect Molecular Biology. 22(1):88-103.
Jones, G., Teal, P.E., Henrich, V., Krzywonos, A., Sapa, A., Wozniak, M., Smolka, J., Jones, D. 2013. Ligand binding pocket function of drosophila USP is necessary for metamorphosis. General and Comparative Endocrinology. 182:73-82.
Nyasembe, V.0., Teal, P.E., Mukabana, V.R., Tumlinson, J.H., Torto, B. 2012. Behavioural response of the malaria vector Anopheles gambiae to host plant volatiles and synthetic blends. Parasites & Vectors. 5:234.
Fombong, A.T., Teal, P.E., Arbogast, R.T., Ndegwa, P.N., Irungu, L.W., Torto, B. 2012. Chemical communication in the honey bee scarab pest Oplostomus haroldi: role of (Z)-9-Pentacosene. Journal of Chemical Ecology. DOI: 10.1007/s10886-012-0211-x.
Christensen, S.A., Nemchenko, A., Borrego, E., Murray, I., Sobhy, I.S., Bosak, L., Deblasio, S., Matthias, E., Christelle, R., Vaughn, K.A., Herrfurth, C., Tumlinson, J., Feussner, I., Jackson, D., Turlings, T., Engelberth, J., Nansen, C., Meeley, R., Kolomiets, M.V. 2013. The maize lipoxygenase, ZmLOX10, mediates green leaf volatile, jasmonate, and herbivore-induced plant volatile production for defense against insect attack. Plant Journal. 74:59-73.
Tchouassi, D.P., Sang, R., Sole, C.L., Bastos, A.D., Teal, P.E., Borgemeister, C., Torto, B. 2013. Common host-derived chemicals increase catches of disease-transmitting mosquitoes and can improve early warning systems for rift valley fever virus. PLOS Neglected Tropical Diseases. 7(1):1-11.
Wang, B., Waters, A.L., Sims, J.W., Fullmer, A., Ellison, S., Hamann, M.T. 2013. Complex marine natural products as potential epigenetic and production regulators of antibiotics from a marine Pseudomonas aeruginosa. Microbial Ecology. 65:1068-1075.
Kachigamba, D.L., Ekesi, S., Ndung'U, M.W., Gitonga, L.M., Teal, P.E., Torto, B. 2012. Evidence for potential of managing some african fruit fly species (Diptera: Tephritidae) using the mango fruit fly host-marking pheromone. Journal of Economic Entomology. 105(6):2068-2075.
Visser, B., Roelofs, D., Hahn, D.A., Teal, P.E., Marien, J., Ellers, J. 2012. Transcriptional changes associated with lack of lipid synthesis in parasitoids. Genome Biology and Evolution. 752-762.
Vaughan, M.M., Webster, F.X., Kiemle, D., Hong, Y.J., Tantillo, D.J., Wang, Q., Coates, R.M., Wray, A.T., Askew, W., O'Donnell, C., Tokuhisa, J.G., Tholl, D. 2012. Formation of the unusual semivolatile Diterpene Rhizathalene by the Arabidopsis Class I Terpene Synthase TPS08 in the root stele is involved in defense against belowground herbivory . The Plant Cell. 1-18.
Fombong, A.T., Mumoki, F.N., Muli, E., Masiga, D.K., Arbogast, R.T., Teal, P.E., Torto, B. 2012. Occurrence, diversity and pattern of damage of Oplostomus species (Coleoptera: Scarabaeidae), honey bee pests in Kenya. Apidologie. DOI: 10.1007/s13592-012-0149-6.