2011 Annual Report
2. Detection and attraction: Develop chemical and acoustic detection and attraction systems for pest species and natural enemies: specifically develop trapping systems using floral-derived volatiles to monitor and/or eliminate pest populations and monitor dispersal of augmented parasitoids, detect acoustic signals produced by cryptic/hidden pests for targeted control, and improve detection efficiency through automation.
3. Biological control: Develop strategies for use of parasitoids and predators in IPM of insect pests through behavioral, ecological and physiological studies of their feeding, mating,dispersal and oviposition: Specifically target conservation biological control on overwintering reservoirs of migratory fall army worm and other pests; develop thelytokous strains of fruit fly parasitoids for augmentative biological control, and develop Asian citrus psyllid diets that will facilitate the mass-production of hosts for mass-rearing parasitoids for augmentative parasitoid releases and other forms of biologically-based control; and develop predictive models of pest migration that incorporate climate-change and facilitate the targeting of vulnerable populations.
To precisely target populations of hidden/cryptic pests distinctive spectral and temporal patterns of acoustic signals will be identified. Insect detectability will be optimized by constructing customized attachments or waveguides connecting the sensors to the substrates. Automated systems can be developed that remotely detect, identify, and count specific pests based on the spectral and temporal patterns of signals. An automated system for trapping C. capitata will consist of a lure and a microphone connected to a signal acquisition andanalysis system attached by cable or wireless to a computer. To develop food/host based attractants for opiine braconid fruit fly parasitoids to monitor augmentative releases and to stimulate oviposition volatiles produced by fruit fly larvae, infested fruits and nectar-sourcesare parasitoids to locate hosts, host-habitats and adult food will be identified and formulated into appropriate lures and additives. To develop floral attractants and pheromones for monitoring and/or control of adult lepidopterous pests research will concentrate on phenylacetaldehyde (PAA) plus ß-myrcene, cis-jasmone, benzyl acetate and additional candidate identified by field surveys. Lures will be first tested in flight tunnels and then in the field.
To develop thelytokous strains of fruit fly parasitoids for augmentative biological control, Wolbachia-infected parasitoid surveys will be based on the theory that asexual populations are more persistent in biologically less diverse environments. To develop improved control strategies for lepidopteran pests that recruit natural enemies, combinations of resistant crops and plants supporting natural enemies will be compared in the field using sentinel plants and a split plot arrangement of treatments. To predict the spatial and temporal shifts in infestations of migratory noctuid pests that result from climate change and to target controls, genetic markers will be used to identify source populations from which migratory pathways can be derived, and historical changes in these pathways explained with meteorological and climatic data.
Nagoshi, R.N. 2011. Artificial selection for developmental rates in fall armyworm (Lepidoptera: Noctuidae) and its implications on the design of feeding studies. Annals of the Entomological Society of America. 104(1):88-94.
Mankin, R.W., Hodges, R.D., Nagle, H.T., Schal, C., Pereira, R.M., Koehler, P.G. 2010. Identification of stored product and urban insect pests by inexpensive infrared, acoustic, and vibrational detection of movement. Journal of Economic Entomology. 103:1636-1646.
Mankin, R.W., Hagstrum, D.W., Smith, M.T., Roda, A.L., Kairo, M.T. 2011. Perspective and Promise: a Century of Insect Acoustic Detection and Monitoring. American Entomologist. 57(1):30-44. Ulyshen, M.D., Mankin, R.W., Chen, Y., Duan, J.J., Poland, T.M., Bauer, L.S. 2011. Role of emerald ash borer (Coleoptera: Buprestidae) larval vibrations in host-quality assessment by Tetrastichus planipennisi (Hymenoptera: Eulophidae). Journal of Economic Entomology. 104(1):81-86.
Sanders, W., Mankin, R.W., Liburd, O., Stelinski, L. 2011. Acoustic detection of arthropod infestation of grape roots: scouting for grape root borer (Lepidoptera:Sesiidae). Florida Entomologist. 94(2):296-302.
Sanders, W.R., Liburd, O.E., Mankin, R.W., Meyer, W.L., Stelinski, L.L. 2011. Applications and mechanisms of wax-based semiochemical dispenser technology for disruption of grape root borer mating. Journal of Economic Entomology. 104(3):939-946.
Roda, A., Kairo, M., Damian, T., Franken, F., Heidweiller, K., Johanns, C., Mankin, R.W. 2011. Red Palm Weevil (Rhynchophorus ferrugineus), an invasive pest recently found in the Caribbean that threatens the region. European Plant Protection Organization Bulletin. 41:116-121.
Raphael, K.A., Streamer, K., Morrow, J.L., Handler, A.M., Frommer, M., Shearman, D.C. 2011. Germ-line transformation of the Queensland fruit fly, Bactrocera tryoni, using a piggyBac vector in the presence of endogenous piggyBac elements. Genetica. 139:91-97.
Schetelig, M.A., Gotschel, F., Viktorinova, I., Handler, A.M., Wimmer, E. 2011. Recombination technologies for enhanced transgene stability in bioengineered insects. Genetica. 139:71-78.
Meza, J., Xavier, N., Zimowska, G.J., Zepeda, C., Handler, A.M. 2011. Development of transgenic strains for the biological control of the Mexican fruit fly, Anastrepha ludens. Genetica. 139:53-62.
Nirmala, X., Olson, S., Holler, T., Cho, K., Handler, A.M. 2011. A DsRed fluorescent protein marker under polyubiquitin promoter regulation allows visual and amplified gene detection of transgenic Caribbean fruit flies in liquid traps. Biocontrol. 56(3):333-340.
Machado, V., Wunder, M., Baldissera, V.D., Oliveria, J.V., Fiuza, L.M., Nagoshi, R.N. 2008. Molecular characterization of host strains of Spodoptera frugiperda(Lepidoptera:Noctuidae)in southern Brazil.Annals of the Entomological Society of America. 101(3):619-626. :. Annals of the Entomological Society of America. 101:619-626.
Nagoshi, R.N. DNA barcoding sequences for Spodoptera ssp. of agricultural importance. National Center for Biotechnology Information (NCBI).Public Database. Accession nos:HM756074-HM756093.
Nagoshi, R.N. 2010. DNA barcoding sequences for Spodoptera frugiperda halotypes. National Center for Biotechnology Information (NCBI).Public Database. Accession nos:HM136586-HMI136602.
Shirk, P.D., Furlong, R.B., Shapiro, J.P. 2009. Species specific ITS-1 DNA Sequences for the identification of Orius insidiosus, Orius pumilio and Orius tristicolor.. National Center for Biotechnology Information (NCBI).Public Database. Acces. No. GU214726-GU214728.
Rohrig, E.A., Shirk, P.D., Hall, D.G., Stansly, P.A. 2011. Larval development of Diaphorencyrtus aligarhensis (Hymenoptera: Encyrtidae) an endoparasitoid of Diaphorina citri (Homoptera: Psyllidae).. Annals of the Entomological Society of America. 104:50-58.
Meagher Jr, R.L., Nagoshi, R.N., Stuhl, C.J. 2011. Oviposition choice of two fall armyworm (Lepidoptera: Noctuidae) host strains. Journal of Insect Behavior. 24(5):337-347.
Pereira, R., Sivinski, J.M., Shapiro, J.P., Teal, P.E. 2011. Influence of methoprene and dietary protein on male Anastrepha suspensa (Diptera: Tephritidae) lipid and protein contents. Florida Entomologist. 94(2):137-144.
Nagoshi, R.N., Fleischer, S., Meagher Jr, R.L. 2009. Texas is the overwintering source of fall armyworm in central Pennsylvainia: implications for migration into the northeastern United States. Journal of Economic Entomology. 38(6):1546-1554.
Shapiro, J.P., Shirk, P.D. 2010. Ovarian development in the predaceous minute pirate bug Orius pumilio: relationship to diet and mating. Annals of the Entomological Society of America. 103(6):971-978.
Shapiro, J.P., Shirk, P.D., Kelley, K., Lewis, T.M., Horton, D.R. 2010. Identity of two sympatric species of Orius (Heteroptera: Anthocoridae). Journal of Insect Science. 10:189.
Sivinski, J.M., Wahl, D., Holler, T., Dobai, S.A., Sivinski, R. 2011. Conserving natural enemies with flowering plants: estimating floral attractiveness to parasitic Hymenoptera and attractions relationship to flower and plant morphology. Biological Control. 58:208-214.
Schetelig, M.A., Xavier, N., Handler, A.M. 2011. Pro-apoptotic cell death genes, hid and reaper, from the tephritid pest species, Anastrepha suspensa. International Congress On Apoptosis. 16(8):759-768.
Schetelig, M.A., Wimmer, E.A. 2011. Insect transgenesis and the sterile insect technique. Book Chapter. II(2):169-194.
Stuhl, C.J., Sivinski, J.M., Teal, P.E., Paranhos, B., Aluja, M. 2011. A compound produced by Fruigivorous Tephritidae (Diptera) larvae promotes oviposition behavior by the biological control agent Diachasmimorpha longicaudata (Hymenoptera: Braconidae). Environmental Entomology. 40(3):727-736.