Systems Biology Investigations of Arthropod-Borne Diseases to Control Vectors of Pathogens
Tick and Biting Fly Research
2012 Annual Report
1a.Objectives (from AD-416):
Emerging infectious diseases (EID) are a threat to global health. Socio-economic, environmental, and ecological factors are believed to drive that increasing health threat. Zoonoses represent the majority of recent EID events. During the last decade vector-borne diseases were shown to comprise 28.8% of the EID events. Arthropods are important vectors of infectious agents pathogenic to humans, and domestic and wild animals. A systems biology approach offers the opportunity to develop effective interventions for the sustainable control of arthropod-borne diseases. Such approach requires the collection and analysis of comprehensive data sets, which enables the integration of components comprising biological systems.
The effects of specific mutations in Borrelia genes that affect disease transmission in a needle inoculation system mouse model will be compared to molecular events occurring during natural infection through the bite of Borrelia-infected Ixodes scapularis. ARS will supply uninfected I. scapularis ticks and expertise on tick biology, while UTSA will conduct the disease transmission studies using mutant strains of Borrelia burgdorferi and evaluate the mutant strain's ability to transmit the disease from infected tick to mouse.
Another objective involves the exploration of phenotypic and genotypic changes on the tick's ability to acquire a blood meal according to its infection status.
The discovery and development of safer acaricides with novel modes of action remains an unmet need. A unique chemical library will be screened for acaricidal activity as part of this collaboration.
1b.Approach (from AD-416):
ARS will establish a colony of Ixodes scapularis ticks and provide adult ticks, larvae, or eggs to cooperator at appropriate stages in the project.
ARS will provide advisory information on I. scapularis biology to guide the experimental design of RNAi experiments seeking to silence specific gene expression of genes that are under consideration as anti-Borrelia vaccine candidates.
Cooperator will provide USDA access to UTSA's chemical library for evaluating the toxicity of the small molecule chemicals to ticks (cattle fever tick, black legged tick, and lone star tick) and biting flies (horn fly, stable fly, and sand fly). UTSA and USDA cooperators will jointly select a group of compounds from the chemical library based on their structures, and UTSA will provide sufficient amount of each selected compound to USDA scientists. The USDA scientists will develop an appropriate bioassay method for each of the target arthropod species that can best utilize the materials. After the initial screening, promising molecules will be identified based on which other related compounds will be selected for the next round of screening.
A PhD student from China was funded in a scientific exchange program with the cooperator. The student completed work in the ARS laboratory on modes of action of four acaricides on ticks. A scientific and technological exchange proposal between the U.S.A. and China to mitigate the impact of tick-borne diseases was prepared jointly by ARS scientists and the university cooperator. The proposal was selected by USDA - Foreign Agricultural Service and China Ministry of Agriculture (MOA). The USDA team made a trip in the summer of 2012 to visit five MOA research institutes in China to further develop research collaborations.