1a.Objectives (from AD-416):
Evaluate potential of myb1A gene constructs (from Vitis vinifera) for use in producing anthocyanin in citrus. If effective, the citrus gene equivalent will be extracted and utilized as a cis-genic selectable marker.
1b.Approach (from AD-416):
Use two constructs obtained from the collaborator in Agrobacterium mediated transformation of citrus and tobacco. Regenerate shoots and assess for presence of myb1A and evaluate gene expression. If promising plants will be grown out for further assessment.
This project is related to Objective 1: Create new genetic combinations of citrus, Objective 2: Screen germplasm for important traits and select superior individuals, Sub objective 1 D: Create new scions and rootstocks with potential resistance to huanglongbing (HLB) and citrus bacterial canker (CBC) by genetic transformation.
Anthocyanin production genes were inserted into citrus and expressed. Transformed shoots have been micrografted and are growing in the greenhouse for further evaluation. Transformation rates with and without the kanamycin selectable marker were evaluated, indicating production of few transgenic shoots when kanamycin was not in the medium.