1a.Objectives (from AD-416)
Evaluate potential of myb1A gene constructs (from Vitis vinifera) for use in producing anthocyanin in citrus. If effective, the citrus gene equivalent will be extracted and utilized as a cis-genic selectable marker.
1b.Approach (from AD-416)
Use two constructs obtained from the collaborator in Agrobacterium mediated transformation of citrus and tobacco. Regenerate shoots and assess for presence of myb1A and evaluate gene expression. If promising plants will be grown out for further assessment.
This project is related to Objective 1: Create new genetic combinations of citrus; Objective 2: Screen germplasm for important traits and select superior individuals; and Subobjective 1 D: Create new scions and rootstocks with potential resistance to Huanglongbing and Citrus Bacterial Canker by genetic transformation.
Anthocyanin production genes have been inserted into citrus and are being expressed. Transformed shoots have been micrografted and are growing in the greenhouse for further evaluation. Transformation rates with and without the kanamycin selectable marker are being evaluated.
Progress was shared with the cooperator through email, phone conversations and in meetings.